Central Division of Analytical Chemistry, Forschungszentrum Jülich, Jülich, Germany.
Metallomics. 2011 Dec;3(12):1271-9. doi: 10.1039/c1mt00116g. Epub 2011 Oct 24.
Metallomics (more specifically, metalloproteomics) is an emerging field that encompasses the role, uptake, transport and storage of trace metals, which are essential to preserve the functions of proteins within a biological system. The current strategies for metal-binding and metalloprotein analysis based on the combination of polyacrylamide gel electrophoresis (PAGE) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) are discussed in this review. The advantages, limitations and the most recently developed and applied quantification approaches for this methodology are also described.
金属组学（更具体地说，金属蛋白质组学）是一个新兴领域，涵盖了痕量金属的作用、摄取、运输和储存，这些金属对于维持生物系统内蛋白质的功能至关重要。本文综述了基于聚丙烯酰胺凝胶电泳（PAGE）和激光烧蚀电感耦合等离子体质谱（LA-ICP-MS）结合的金属结合和金属蛋白分析的当前策略。还描述了该方法的优点、局限性以及最近开发和应用的定量方法。
