School of Environment, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, 310024, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Anal Bioanal Chem. 2022 Oct;414(24):7023-7033. doi: 10.1007/s00216-022-04185-2. Epub 2022 Jul 5.
New analytical strategies for metal-binding protein facilitate researchers learning about how metals play a significant role in life. Laser ablation-inductively coupled plasma mass spectrometry (LA-ICP-MS) offers many advantages for the metal analysis of biological samples and shows a promising future in protein analysis, but recent advances in LA-ICP-MS-based strategies for identifying metal-binding proteins via endogenous metals remain less updated yet. To present the current status in this field, the main analytical strategies for metal-binding proteins with LA-ICP-MS are reviewed here, including in situ analysis of biospecimens and ex situ analysis with gel electrophoresis. A critical discussion of challenges and future perspectives is also given. Multifarious laser ablation-inductively coupled plasma mass spectrometry (LA-ICP-MS)-based strategies have been developed and applied to investigate the metal-binding proteins in biospecimens in situ or through gel electrophoresis ex situ over the past decades, facilitating researchers disclosing how essential metals are implicated in life or what proteins toxic metals will target.
新的金属结合蛋白分析策略有助于研究人员了解金属在生命中发挥的重要作用。激光烧蚀-电感耦合等离子体质谱（LA-ICP-MS）为生物样品的金属分析提供了许多优势，并且在蛋白质分析方面具有广阔的前景，但最近在基于 LA-ICP-MS 的策略方面，通过内源性金属来鉴定金属结合蛋白的进展仍然较少。为了介绍该领域的现状，本文综述了用于 LA-ICP-MS 的金属结合蛋白的主要分析策略，包括生物标本的原位分析和凝胶电泳的非原位分析。还对挑战和未来展望进行了批判性讨论。在过去的几十年中，已经开发并应用了多种基于激光烧蚀-电感耦合等离子体质谱（LA-ICP-MS）的策略，用于原位或通过凝胶电泳非原位研究生物标本中的金属结合蛋白，从而有助于研究人员揭示生命中必需的金属是如何涉及的，或者有毒金属将针对哪些蛋白质。
