Qβ 和 MS2 正链 RNA 噬菌体复制酶介导的负链 RNA 复制
Negative-strand RNA replication by Q beta and MS2 positive-strand RNA phage replicases.
作者信息
Shaklee P N
机构信息
Department of Biochemistry, Texas College of Osteopathic Medicine/University of North Texas, Fort Worth 76107.
出版信息
Virology. 1990 Sep;178(1):340-3. doi: 10.1016/0042-6822(90)90417-p.
PMID:2202149
Abstract
Plasmids were constructed which allowed preparation of positive- and negative-strand MS2 RNA by in vitro transcription and which expressed the MS2 phage replicase. Transcribed, positive-strand MS2 RNA was infectious to Escherichia coli spheroplasts. Transcribed, negative-strand MS2 RNA was infectious to spheroplasts containing either MS2 replicase or related bacteriophage Q beta replicase.
摘要
构建了质粒，可通过体外转录制备正链和负链MS2 RNA，并表达MS2噬菌体复制酶。转录的正链MS2 RNA对大肠杆菌原生质球具有感染性。转录的负链MS2 RNA对含有MS2复制酶或相关噬菌体Qβ复制酶的原生质球具有感染性。
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