RNA复制酶β（MS2噬菌体）与DNA聚合酶（T7噬菌体和大肠杆菌）弱同源氨基酸序列的计算机辅助检测与比对

Computer-aided detection and alignment of weakly homologous amino acid sequences of RNA replicase beta (MS2 phage) and DNA polymerases (T7 phage and E. coli).

作者信息

Ohnishi K

机构信息

Department of Biology, Faculty of Science, Niigata University, Japan.

出版信息

Nucleic Acids Symp Ser. 1988(19):193-7.

PMID:3067216

Abstract

Alignment of the amino acid (aa) sequences of T7 phage DNA polymerase (DPase), E. coli DNA polymerase I (Pol I) and MS2 phage RNA replicase beta subunit (MS2 Repl) were established by computer-aided methods. The results showed that the entire length (aa's 16-704) of T7 DPase is homologous to Pol I aa's 207-928(C-term) with 21.5% aa identity, and that domains I (aa's-1-311) and II (312-451(C-term] were found to be homologous to each other and to N-terminal region of T7 DPase (aa's 1-250). Thus these enzymes and domains are homologous to one another and must have evolved from a co-ancestral enzyme.

摘要

通过计算机辅助方法对T7噬菌体DNA聚合酶（DPase）、大肠杆菌DNA聚合酶I（Pol I）和MS2噬菌体RNA复制酶β亚基（MS2 Repl）的氨基酸（aa）序列进行了比对。结果表明，T7 DPase的全长（氨基酸16 - 704）与Pol I的氨基酸207 - 928（C端）同源，氨基酸同一性为21.5%，并且发现结构域I（氨基酸 - 1 - 311）和结构域II（312 - 451（C端））彼此同源，且与T7 DPase的N端区域（氨基酸1 - 250）同源。因此，这些酶和结构域彼此同源，必定是从共同的祖先酶进化而来的。