Swanson P E, Lillemoe T J, Manivel J C, Wick M R
Department of Laboratory Medicine and Pathology, University of Minnesota School of Medicine, Minneapolis.
Arch Pathol Lab Med. 1990 Sep;114(9):943-8.
Mesenchymal chondrosarcoma is an uncommon small-cell neoplasm of bone and soft tissue, the chondrogenic nature of which has been generally accepted. However, the phenotypic attributes of the small-cell population in this neoplasm have not been well characterized, and its relationship to "precartilage mesenchyme" remains unclear. In an attempt to address this issue, we performed an immunohistochemical analysis of nine cases, using antibodies to vimentin, S100 protein, Leu-7 antigen, neuron-specific enolase, synaptophysin, desmin, muscle-specific actin, cytokeratin, and epithelial membrane antigen, and the avidin-biotin-peroxidase complex (ABC) method. The small cells of mesenchymal chondrosarcoma failed to express S100 protein, whereas all components of the tumors (small cells, lacunar chondroblasts, and chondroid matrix) stained for Leu-7 antigen in six cases. Neuron-specific enolase was identified in the small cells of four cases and in the lacunar cells of seven. None contained desmin, actin, cytokeratin, epithelial membrane antigen, or synaptophysin. The immunophenotype of mesenchymal chondrosarcoma resembled that of embryonic cartilage and thus did not contradict the premise that this tumor was the neoplastic counterpart of fetal chondroid tissues. However, immunohistologic studies are not overly helpful in the differential diagnosis between mesenchymal chondrosarcoma and other small round cell lesions.
间叶性软骨肉瘤是一种罕见的骨与软组织小细胞肿瘤，其软骨形成性质已得到普遍认可。然而，该肿瘤中小细胞群体的表型特征尚未得到充分描述，其与“软骨前间充质”的关系仍不明确。为解决这一问题，我们使用波形蛋白、S100蛋白、Leu-7抗原、神经元特异性烯醇化酶、突触素、结蛋白、肌肉特异性肌动蛋白、细胞角蛋白和上皮膜抗原的抗体以及抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法，对9例病例进行了免疫组织化学分析。间叶性软骨肉瘤的小细胞未表达S100蛋白，而在6例病例中，肿瘤的所有成分（小细胞、陷窝软骨母细胞和软骨样基质）均被Leu-7抗原染色。在4例病例的小细胞和7例病例的陷窝细胞中发现了神经元特异性烯醇化酶。均未含有结蛋白、肌动蛋白、细胞角蛋白、上皮膜抗原或突触素。间叶性软骨肉瘤的免疫表型类似于胚胎软骨，因此并不与该肿瘤是胎儿软骨样组织的肿瘤对应物这一前提相矛盾。然而，免疫组织学研究在间叶性软骨肉瘤与其他小圆形细胞病变的鉴别诊断中帮助不大。
