Calderhead D M, Kitagawa K, Lienhard G E, Gould G W
Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03756.
Biochem J. 1990 Aug 1;269(3):597-601. doi: 10.1042/bj2690597.
Insulin-stimulated glucose transport was examined in BC3H-1 myocytes. Insulin treatment lead to a 2.7 +/- 0.3-fold increase in the rate of deoxyglucose transport and, under the same conditions, a 2.1 +/- 0.1-fold increase in the amount of the brain-type glucose transporter (GLUT 1) at the cell surface. It has been shown that some insulin-responsive tissues express a second, immunologically distinct, transporter, namely GLUT 4. We report here that BC3H-1 myocytes and C2 and G8 myotubes express only GLUT 1; in contrast, rat soleus muscle and heart express 3-4 times higher levels of GLUT 4 than GLUT 1. Thus translocation of GLUT 1 can account for most, if not all, of the insulin stimulation of glucose transport in BC3H-1 myocytes. On the other, hand, neither BC3H-1 myocytes nor the other muscle-cell lines are adequate as models for the study of insulin regulation of glucose transport in muscle tissue.
在BC3H-1肌细胞中检测了胰岛素刺激的葡萄糖转运。胰岛素处理使脱氧葡萄糖转运速率增加了2.7±0.3倍，在相同条件下，细胞表面脑型葡萄糖转运蛋白（GLUT 1）的量增加了2.1±0.1倍。已表明一些胰岛素反应性组织表达第二种免疫上不同的转运蛋白，即GLUT 4。我们在此报告，BC3H-1肌细胞以及C2和G8肌管仅表达GLUT 1；相反，大鼠比目鱼肌和心脏中GLUT 4的表达水平比GLUT 1高3至4倍。因此，GLUT 1的易位可以解释BC3H-1肌细胞中胰岛素刺激葡萄糖转运的大部分（如果不是全部）。另一方面，BC3H-1肌细胞和其他肌肉细胞系都不足以作为研究肌肉组织中胰岛素对葡萄糖转运调节的模型。
