Departamento de Biologia, Laboratório de Biologia Celular, UNESP-IBILCE, São José do Rio Preto, SP, Brazil.
Micron. 2012 Feb;43(2-3):298-304. doi: 10.1016/j.micron.2011.09.004. Epub 2011 Sep 18.
Mg(2+)-dependent ATPases were investigated in Malpighian tubules of the blood-sucking insect, Triatoma infestans, with cytochemical procedures for light and electron microscopy. The aim was to establish patterns of enzyme occurrence in the blood-sucking insect under control rearing conditions for further comparisons with animals subjected to the action of stress factors. Enzyme activity was found in laminated "concretions" present in distal cells, in edges of urate crystals at the lumen of the proximal region of tubules, in the basement membrane of proximal cells, and variously distributed in plasmalemma invaginations of both distal and proximal cells. Presence of ATPases in the "concretions" and urate crystals is presumed to be due to engulfment of other ATPase-containing components during formation of these structures. Cytochemical reactivity in the basement membrane and plasmalemma invaginations is assumed to be involved with active transport of waste molecules from and to hemolymph and differs as a function of the Malpighian tubule region. This paper provides a basic understanding of the enzyme occurrence in the blood sucking insects, and can be used as a pattern for comparative means of the staining patterns among Triatominae species.
使用细胞化学方法对吸血昆虫 Triatoma infestans 的马氏管进行了研究，以研究 Mg(2+)-依赖性 ATP 酶。目的是确定在吸血昆虫的控制饲养条件下酶的出现模式，以便与受到应激因素作用的动物进行进一步比较。在远侧细胞的层状“结石”、在近端区域的管腔中尿酸晶体的边缘、在近端细胞的基底膜中以及远侧和近端细胞的质膜内陷中均发现了酶活性。“结石”和尿酸晶体中 ATP 酶的存在被认为是由于在形成这些结构期间吞噬了其他含 ATP 酶的成分。基底膜和质膜内陷中的细胞化学反应被认为与废物分子从血淋巴和向血淋巴的主动运输有关，并且根据马氏管区域而不同。本文提供了对吸血昆虫中酶出现的基本了解，并可用作比较 Triatominae 物种染色模式的基础。
