de Azeredo-Oliveira M T, Mello M L
Department of Biology, IBILCE, UNESP, São José do Rio Preto, Brazil.
Cytobios. 1998;93(373):83-92.
Benzidine and diamino benzidine (DAB) oxidation, typically performed by peroxidases, was demonstrated by light and electron microscopy in peroxisomes, mitochondria and membranous structures which occurred in close contact with urate crystals in Malpighian tubules of nymphs and adults of Triatoma infestans. Peroxisomes were predominantly identified in cells of the distal region of the tubules, which is engaged in excretory mechanisms. DAB oxidation in mitochondria, even in the absence of hydrogen peroxide, may indicate the existence of a mitochondrial peroxidase and possibly a cytochrome c peroxidase. The localization of the extracellular membranous structures appeared restricted to the lumen of the proximal region of the tubules and they were assumed to be remnants of endoplasmic reticulum containing peroxidases.
联苯胺和二氨基联苯胺（DAB）氧化反应通常由过氧化物酶催化，通过光镜和电镜观察发现，在骚扰锥蝽若虫和成虫的马氏管中，过氧化物酶体、线粒体以及与尿酸盐晶体紧密接触的膜状结构内均存在该反应。过氧化物酶体主要在马氏管远端区域的细胞中被鉴定出来，该区域参与排泄机制。即使在没有过氧化氢的情况下，线粒体中的DAB氧化反应也可能表明存在线粒体过氧化物酶，甚至可能存在细胞色素c过氧化物酶。细胞外膜状结构的定位似乎局限于马氏管近端区域的管腔，推测它们是含有过氧化物酶的内质网残余物。
