School of Chemistry and Chemical Engineering, Anhui University, Hefei 230039, China.
Biosens Bioelectron. 2012 Jan 15;31(1):544-7. doi: 10.1016/j.bios.2011.10.001. Epub 2011 Oct 12.
A novel enzymatic hydrogen peroxide sensor was successfully fabricated based on the nanocomposites containing of Ag/C nanocables and gold nanoparticles (AuNPs). Ag/C nanocables have been synthesized by a hydrothermal method and then AuNPs were assembled on the surface of Ag/C nanocables. The nanocomposites were confirmed by X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometry (EDS). The above nanocomposites have satisfactory chemical stability and excellent biocompatibility. Cyclic voltammetry (CV) was used to evaluate the electrochemical performance of the Ag/C/Au nanocomposites at glassy carbon electrode (GCE). The results indicated that the Ag/C/Au nanocomposites exhibited excellent electrocatalytic activity to the reduction of H(2)O(2). It offered a linear range of 6.7×10(-9) to 8.0×10(-6) M, with a detection limit of 2.2×10(-9) M. The apparent Michaelis-Menten constant of the biosensor was 51.7×10(-6) M. These results indicated that Ag/C/Au nanocomposites have potential for constructing of a variety of electrochemical biosensors.
一种新型的酶促过氧化氢传感器是基于含有 Ag/C 纳米电缆和金纳米粒子（AuNPs）的纳米复合材料成功制备的。Ag/C 纳米电缆是通过水热法合成的，然后 AuNPs 被组装在 Ag/C 纳米电缆的表面上。通过 X 射线衍射（XRD）、透射电子显微镜（TEM）、扫描电子显微镜（SEM）和能谱（EDS）对纳米复合材料进行了确认。上述纳米复合材料具有良好的化学稳定性和优异的生物相容性。循环伏安法（CV）用于评估玻璃碳电极（GCE）上 Ag/C/Au 纳米复合材料的电化学性能。结果表明，Ag/C/Au 纳米复合材料对 H（2）O（2）的还原具有优异的电催化活性。它提供了 6.7×10（-9）至 8.0×10（-6）M 的线性范围，检测限为 2.2×10（-9）M。生物传感器的表观米氏常数为 51.7×10（-6）M。这些结果表明，Ag/C/Au 纳米复合材料具有构建各种电化学生物传感器的潜力。
