Département des Neurosciences Fondamentales, University of Geneva, Switzerland.
Physiol Meas. 2011 Nov;32(11):N33-53. doi: 10.1088/0967-3334/32/11/N01. Epub 2011 Oct 26.
Laser-Doppler flowmetry (LDF) is an outstanding tool to monitor blood flow in a continuous and non-invasive way. In this work, we study LDF at large interoptode spacing applied to a human bone model (i.e. tibia diaphysis). To that aim, we first performed an extensive set of Monte Carlo (MC) simulations for 10 and 25 mm interoptode spacing. Second, we have assembled a dedicated LDF instrumentation based on an optimized industrial avalanche photo-detector. We performed LDF experimental measurements on human muscle using well-known physiological protocols, which confirmed the reliability of our instrumentation and the relevance of the LDF algorithms tested with the MC simulations. In a second set, we repeated the measurements on human tibia diaphysis. Again, the experiments corroborate the MC simulations and demonstrate the effectiveness of LDF to monitor blood perfusion in bone. The proposed technique has great potential for non-invasive neuro-vascular studies since it will certainly help to reveal the mechanisms underlying the interaction between bone/bone marrow, the circulatory system and the nervous system.
激光多普勒流量metry（LDF）是一种出色的工具，可用于连续且非侵入性地监测血流。在这项工作中，我们研究了应用于人体骨骼模型（即胫骨骨干）的大光极间距的 LDF。为此，我们首先针对 10 和 25mm 光极间距进行了广泛的蒙特卡罗（MC）模拟。其次，我们基于优化的工业雪崩光电探测器组装了专用的 LDF 仪器。我们使用众所周知的生理协议在人体肌肉上进行了 LDF 实验测量，这证实了我们的仪器的可靠性以及与 MC 模拟一起测试的 LDF 算法的相关性。在第二组中，我们在人体胫骨骨干上重复了测量。同样，实验证实了 MC 模拟，并证明了 LDF 监测骨骼中血液灌注的有效性。该技术具有很大的非侵入性神经血管研究潜力，因为它肯定有助于揭示骨骼/骨髓，循环系统和神经系统之间相互作用的机制。
