Kornreich B G, Craven M, McDonough S P, Nydam D V, Scorza V, Assarasakorn S, Lappin M, Simpson K W
Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, USA. (
J Comp Pathol. 2012 May;146(4):298-307. doi: 10.1016/j.jcpa.2011.07.006. Epub 2011 Oct 24.
Bacterial endocarditis (BE) is defined as inflammation of cardiac valve structures and/or the endocardium secondary to bacterial infection. Canine valvular BE is associated with significant morbidity and mortality and ante-mortem diagnosis and post-mortem identification of causative organisms is problematic. Identification of bacteria in canine BE has traditionally relied on visualization of organisms on histological sections stained with haematoxylin and eosin (HE), Gram and modified Steiner's stains. Each of these staining techniques has limitations with respect to identification of bacterial species in cases of BE. Fluorescence in-situ hybridization (FISH) has been introduced recently as a technique to identify bacteria in biological specimens. To our knowledge, FISH has not been used previously to identify bacteria in archival samples of heart valves from dogs with naturally occurring BE. We sought to determine whether FISH could detect the presence and species of bacteria in archival heart valve sections from dogs with BE, and to compare FISH to histochemical stains in the identification of bacteria. FISH detected bacteria in seven of 17 cases of canine BE and showed near perfect agreement with modified Steiner's stain for the detection of bacteria. FISH identified Streptococcus spp. and/or Staphylococcus spp. in all of these cases, but Bartonella spp. were not identified.
细菌性心内膜炎（BE）被定义为继发于细菌感染的心脏瓣膜结构和/或心内膜的炎症。犬瓣膜性BE与显著的发病率和死亡率相关，生前诊断和死后确定病原体存在问题。传统上，犬BE中细菌的鉴定依赖于在苏木精和伊红（HE）、革兰氏和改良施泰纳氏染色的组织切片上观察病原体。这些染色技术中的每一种在BE病例中细菌种类的鉴定方面都有局限性。荧光原位杂交（FISH）最近作为一种鉴定生物标本中细菌的技术被引入。据我们所知，FISH以前尚未用于鉴定自然发生BE的犬心脏瓣膜存档样本中的细菌。我们试图确定FISH是否能检测出BE犬心脏瓣膜存档切片中细菌的存在和种类，并将FISH与组织化学染色在细菌鉴定方面进行比较。FISH在17例犬BE中的7例中检测到了细菌，并且在检测细菌方面与改良施泰纳氏染色显示出近乎完美的一致性。FISH在所有这些病例中都鉴定出了链球菌属和/或葡萄球菌属，但未鉴定出巴尔通体属。
