Department of Surgery, Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614, United States.
Carbohydr Res. 2011 Dec 13;346(17):2752-9. doi: 10.1016/j.carres.2011.09.029. Epub 2011 Oct 2.
The Candida albicans cell wall provides an architecture that allows for the organism to survive environmental stress as well as interaction with host tissues. Previous work has focused on growing C. albicans on media such as Sabouraud or YPD at 30°C. Because C. albicans normally colonizes a host, we hypothesized that cultivation on blood or serum at 37°C would result in structural changes in cell wall mannan. C. albicans SC5314 was inoculated onto YPD, 5% blood, or 5% serum agar media three successive times at 30°C and 37°C, then cultivated overnight at 30°C in YPD. The mannan was extracted and characterized using 1D and 2D (1)H NMR techniques. At 30°C cells grown in blood and serum contain less acid-stable terminal β-(1→2)-linked d-mannose and α-(1→2)-linked d-mannose-containing side chains, while the acid-labile side chains of mannan grown in blood and serum contain fewer β-Man-(1→2)-α-Man-(1→ side chains. The decrement in acid-stable mannan side chains is greater at 37°C than at 30°C. Cells grown on blood at 37°C show fewer →6)-α-Man-(1→ structural motifs in the acid-stable polymer backbone. The data indicate that C. albicans, grown on media containing host-derived components, produces less complex mannan. This is accentuated when the cells are cultured at 37°C. This study demonstrates that the C. albicans cell wall is a dynamic and adaptive organelle, which alters its structural phenotype in response to growth in host-derived media at physiological temperature.
白色念珠菌细胞壁提供了一种架构，使生物体能够在环境压力以及与宿主组织相互作用下生存。以前的工作主要集中在 30°C 下在萨布罗琼脂或 YPD 等培养基上培养白色念珠菌。由于白色念珠菌通常在宿主中定植，我们假设在 37°C 的血液或血清中培养会导致细胞壁甘露聚糖的结构发生变化。将白色念珠菌 SC5314 连续三次接种到 YPD、5%血液或 5%血清琼脂培养基中，分别在 30°C 和 37°C 下培养，然后在 30°C 的 YPD 中培养过夜。使用 1D 和 2D (1)H NMR 技术提取和表征甘露聚糖。在 30°C 下，在血液和血清中生长的细胞含有较少的酸稳定末端β-(1→2)-连接的 d-甘露糖和α-(1→2)-连接的含 d-甘露糖的侧链，而在血液和血清中生长的甘露聚糖的酸不稳定侧链含有较少的β-Man-(1→2)-α-Man-(1→侧链。在 37°C 下，酸稳定甘露聚糖侧链的减少量大于在 30°C 下。在 37°C 下在血液中生长的细胞在酸稳定聚合物主链中显示出较少的→6)-α-Man-(1→结构基序。数据表明，在含有宿主来源成分的培养基中生长的白色念珠菌产生较少复杂的甘露聚糖。当细胞在生理温度下在宿主来源的培养基中培养时，这种情况更加明显。本研究表明，白色念珠菌细胞壁是一种动态和适应性的细胞器，它会根据在宿主来源的培养基中生长的情况改变其结构表型。
