[山豆根致大鼠肝毒性的机制研究]

[Mechanism studies on hepatotoxicity of rats induced by Sophorae Tonkinensis Radix et Rhizoma in rat].

作者信息

Li Fengjie, Yao Guangtao, Jin Ruomin, Song Zheping

机构信息

Center for Drug Safety Evaluation, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2011 Jul;36(13):1821-3.

PMID:22032152

Abstract

OBJECTIVE

To study the hepatotoxicity mechanism of rats that induced by Sophorae Tonkinensis Radix et Rhizoma.

METHOD

The SD rats were randomly divided into the control and Sophorae Tonkinensis Radix et Rhizoma (RRST) group, given distilled water and RRST 10 g x kg(-1) separately by orally for 7 days, and RRST 20 g x kg(-1) for other days until the 26th day. Blood was drawn from abdominal aorta after the rats were anesthetized by 25% urethane, and then centrifugally processed to get the serum for detection of ALT and AST. The liver tissues of control and experimental group were taken to prepare liver homogenate with cold NS, and centrifugally processed to get the supernatant. The activities of SOD and GSH, the content of gamma-GT and MDA were detected according to the methods of kit. The tumor necosis factor(TNF-alpha) was detected by ABC-ELISA. The expression of ICAM-1 was detected by immunohistochemistry.

RESULT

After the rats were given RRST by oral for 26 days, the ALT and AST activities in serum increased, the content of GSH and the ratio of SOD and MDA decreased, the content of gamma-GT and TNF-alpha, the masculine expression of ICAM-1 increased.

CONCLUSION

After the rats were given RRST, the liver can be damaged obviously, and the mechanism of hepatotoxicity perhaps related to free radical and inflammatory factor.

摘要

目的

研究山豆根诱导大鼠肝毒性的机制。

方法

将SD大鼠随机分为对照组和山豆根组，分别给予蒸馏水和10 g·kg⁻¹山豆根口服7天，之后给予20 g·kg⁻¹山豆根直至第26天。用25%乌拉坦麻醉大鼠后从腹主动脉取血，离心后获取血清检测谷丙转氨酶（ALT）和谷草转氨酶（AST）。取对照组和实验组肝脏组织用冷生理盐水制备肝匀浆，离心后取上清液。按照试剂盒方法检测超氧化物歧化酶（SOD）和谷胱甘肽（GSH）活性、γ-谷氨酰转肽酶（γ-GT）和丙二醛（MDA）含量。用ABC-ELISA法检测肿瘤坏死因子（TNF-α）。用免疫组织化学法检测细胞间黏附分子-1（ICAM-1）的表达。