Univ. Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
Biosens Bioelectron. 2012 Jan 15;31(1):62-8. doi: 10.1016/j.bios.2011.09.035. Epub 2011 Oct 2.
A new electropolymerizable monomer, [N-(6-(4-hydroxy-6-isopropylamino-1,3,5-triazin-2-ylamino)hexyl) 5-hydroxy-1,4-naphthoquinone-3-propionamide], has been designed for use in a label-free electrochemical immunosensor when polymerized on an electrode and coupled with a monoclonal anti-atrazine antibody. This monomer contains three functional groups: hydroxyl group for electropolymerization, quinone group for its transduction capability, and hydroxyatrazine as bioreceptor element. Square wave voltammetry shows that the polymer film, poly[N-(6-(4-hydroxy-6-isopropylamino-1,3,5-triazin-2-ylamino)hexyl) 5-hydroxy-1,4-naphthoquinone-3-propionamide], presents negative current change following anti-atrazine antibody complexation and positive current change after atrazine addition in solution. This constitutes a direct, label-free and signal-on electrochemical immunosensor, with a very low detection limit of ca. 1 pM, i.e. 0.2 ng L(-1), one of the lowest reported for such immunosensors. This is far lower than the detection limit required by the European Union directives for drinkable water and food samples (0.1 μg L(-1)). The strategy described has great promise for the development of simple, cost-effective and reagentless on-site environmental monitors.
一种新的可电化学聚合的单体，[N-(6-(4-羟基-6-异丙基氨基-1,3,5-三嗪-2-基氨基)己基)5-羟基-1,4-萘醌-3-丙酰胺]，已被设计用于在电极上聚合并与单克隆抗阿特拉津抗体偶联的无标记电化学免疫传感器中使用。该单体包含三个功能基团：用于电化学聚合的羟基、用于其转导能力的醌基和作为生物受体元件的羟基阿特拉津。方波伏安法显示，聚合物膜，聚[N-(6-(4-羟基-6-异丙基氨基-1,3,5-三嗪-2-基氨基)己基)5-羟基-1,4-萘醌-3-丙酰胺]，在与抗阿特拉津抗体复合物结合后呈现负电流变化，而在溶液中加入阿特拉津后呈现正电流变化。这构成了一种直接、无标记和信号开启的电化学免疫传感器，检测限非常低，约为 1 pM，即 0.2 ng L(-1)，是此类免疫传感器中报道的最低检测限之一。这远低于欧盟饮用水和食品样本指令要求的检测限（0.1 μg L(-1)）。所描述的策略为开发简单、经济高效且无需试剂的现场环境监测器提供了广阔的前景。
