Università degli Studi di Milano, Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Via Celoria 10, 20133 Milano, Italy.
Vet J. 2012 Jun;192(3):514-6. doi: 10.1016/j.tvjl.2011.08.018. Epub 2011 Oct 26.
MicroRNAs (miRNAs) are a family of regulatory molecules involved in many physiological processes, including activation of cells of the immune system. This study investigated the effect of Escherichia coli lipopolysaccharide (LPS) and Staphylococcus aureus enterotoxin B (SEB) on the expression of five miRNAs involved in the inflammatory response, including miR-9, miR-125 b, miR-155, miR-146 a and miR-223, in bovine CD14(+) cells (monocytes). Incubation of monocytes with SEB induced down-regulation of miR-155, miR-223 and miR-125 b, but not the anti-inflammatory miRNA miR-146 a. Conversely, incubation with LPS upregulated both miR-155 and miR-146 a. In vitro incubation of isolated CD14(+) bovine monocytes with LPS and SEB elicited different and opposite expression of miRNAs reportedly involved in inflammatory reactions.
微小 RNA（miRNAs）是一类参与多种生理过程的调控分子，包括免疫系统细胞的激活。本研究探讨了大肠杆菌脂多糖（LPS）和金黄色葡萄球菌肠毒素 B（SEB）对参与炎症反应的五种 miRNAs 的表达的影响，包括 miR-9、miR-125b、miR-155、miR-146a 和 miR-223，在牛 CD14（+）细胞（单核细胞）中。用 SEB 孵育单核细胞可下调 miR-155、miR-223 和 miR-125b，但不影响抗炎性 miRNA miR-146a。相反，用 LPS 孵育可上调 both miR-155 和 miR-146a。体外孵育分离的 CD14（+）牛单核细胞用 LPS 和 SEB 引发了据称参与炎症反应的 miRNAs 的不同和相反的表达。
