Karlsruhe Institute of Technology (KIT), Institute for Microstructure Technology, 76344 Eggenstein-Leopoldshafen, Germany.
Anal Biochem. 2012 Jan 15;420(2):188-90. doi: 10.1016/j.ab.2011.10.006. Epub 2011 Oct 8.
Analyte detection with biosensors is strongly influenced by the preparation of the biosensor surface including choice of sensing layers and coupling methods for corresponding capture molecules. We investigated the influence of different coupling procedures, especially considering coupling chemistry and incubation times for reagents, by means of surface acoustic wave (SAW) biosensors. The effect on the signal response was tested in two subsequent protein assays. Our optimized coupling procedure allowed the detection of the breast cancer markers HER-2 (human epidermal growth factor receptor-2) and TIMP-1 (tissue inhibitor of metalloproteinase-1) below the respective clinical cutoff values of only a few nanograms per milliliter.
生物传感器中的分析物检测受到生物传感器表面制备的强烈影响，包括传感层的选择和相应捕获分子的偶联方法。我们通过表面声波（SAW）生物传感器研究了不同偶联程序的影响，特别是考虑了偶联化学和试剂的孵育时间。我们在随后的两种蛋白质检测中测试了这种方法对信号响应的影响。我们优化的偶联程序能够检测到乳腺癌标志物 HER-2（人表皮生长因子受体-2）和 TIMP-1（基质金属蛋白酶抑制剂-1），其检测限低至每毫升几个纳克，低于各自的临床检测限。
