Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR, Videnska 1083, 142 20 Prague, Czech Republic.
Free Radic Res. 2012 Apr;46(4):492-522. doi: 10.3109/10715762.2011.632415. Epub 2011 Nov 9.
Oxidatively damaged DNA is implicated in various diseases, including neurodegenerative disorders, cancer, diabetes, cardiovascular and inflammatory diseases as well as aging. Several methods have been developed to detect oxidatively damaged DNA. They include chromatographic techniques, the Comet assay, (32)P-postlabelling and immunochemical methods that use antibodies to detect oxidized lesions. In this review, we discuss the detection of 8-oxo-7,8-dihydro-29-deoxyguanosine (8-oxodG), the most abundant oxidized nucleoside. This lesion is frequently used as a marker of exposure to oxidants, including environmental pollutants, as well as a potential marker of disease progression. We concentrate on studies published between the years 2000 and 2011 that used enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry to detect 8-oxodG in humans, laboratory animals and in cell lines. Oxidative damage observed in these organisms resulted from disease, exposure to environmental pollutants or from in vitro treatment with various chemical and physical factors.
氧化损伤的 DNA 与多种疾病有关，包括神经退行性疾病、癌症、糖尿病、心血管疾病和炎症性疾病以及衰老。已经开发了几种方法来检测氧化损伤的 DNA。它们包括色谱技术、彗星分析、（32）P-后标记和免疫化学方法，这些方法使用抗体来检测氧化损伤。在这篇综述中，我们讨论了 8-氧代-7,8-二氢-29-脱氧鸟苷（8-oxodG）的检测，这是最丰富的氧化核苷。这种损伤经常被用作暴露于氧化剂的标志物，包括环境污染物，以及疾病进展的潜在标志物。我们集中讨论了 2000 年至 2011 年期间发表的使用酶联免疫吸附测定（ELISA）和免疫组织化学检测人类、实验动物和细胞系中 8-oxodG 的研究。这些生物体中观察到的氧化损伤是由疾病、暴露于环境污染物或体外用各种化学和物理因素处理引起的。
