Mustafa Nachvak S, Reza Neyestani T, Ali Mahboob S, Sabour S, Ali Keshawarz S, Speakman J R
1] Faculty of Public Health, Kermanshah University of Medical Sciences, Kermanshah, Iran [2] Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, UK.
National Nutrition and food technology Research Institute (NNFTRI), Iran.
Eur J Clin Nutr. 2014 Oct;68(10):1119-23. doi: 10.1038/ejcn.2014.97. Epub 2014 Jun 18.
Down syndrome (DS) is the most common human chromosomal abnormality. It is characterized by mental retardation and several metabolic disturbances, including elevated oxidative stress, which may be causally linked. Treatment with dietary antioxidants has been suggested as a potential method to alleviate the oxidative damage and retardation of DS patients, but prior supplementation work has been equivocal.
To evaluate the effects of supplementation with antioxidants α-tocopherol and α-lipoic acid (ALA) on oxidative stress biomarkers in DS children.
Ninety-three DS children aged 7-15 years from both sexes were randomly allocated to three groups: α-tocopherol (400 IU/day), ALA (100 mg/day) and placebo. The intervention period was 4 months. A healthy control group consisted 26 non-DS siblings. Serum thiobarbituric acid reactive substances (TBARS) and urinary 8-hydroxy-2'-deoxyguanosine (8OHdG) were used as biomarkers of oxidative stress.
DS children had greater levels of baseline oxidative stress than their siblings. Moreover, males had greater levels of 8OHdG than females (P<0.001) but there was no significant association between age and biomarkers of oxidative stress. Serum levels of TBARS did not change significantly over time, or relative to placebo. Although urinary 8OHdG concentrations decreased significantly in both α-tocopherol and ALA, groups compared with the baseline levels (P<0.001), mean final levels of urinary 8OHdG concentrations differed significantly only between α-tocopherol and placebo groups (P<0.01).
α-Tocopherol supplementation of the diets of DS children may attenuate oxidative stress at the DNA level.
唐氏综合征（DS）是最常见的人类染色体异常疾病。其特征为智力发育迟缓以及多种代谢紊乱，包括氧化应激升高，二者可能存在因果联系。有人提出用膳食抗氧化剂进行治疗是减轻DS患者氧化损伤和智力发育迟缓的一种潜在方法，但之前的补充剂研究结果并不明确。
评估补充抗氧化剂α-生育酚和α-硫辛酸（ALA）对DS儿童氧化应激生物标志物的影响。
将93名7至15岁的DS儿童随机分为三组：α-生育酚组（400国际单位/天）、ALA组（100毫克/天）和安慰剂组。干预期为4个月。一个健康对照组由26名非DS的兄弟姐妹组成。血清硫代巴比妥酸反应性物质（TBARS）和尿8-羟基-2'-脱氧鸟苷（8OHdG）用作氧化应激的生物标志物。
DS儿童的基线氧化应激水平高于其兄弟姐妹。此外，男性的8OHdG水平高于女性（P<0.001），但年龄与氧化应激生物标志物之间无显著关联。血清TBARS水平随时间或相对于安慰剂均无显著变化。虽然与基线水平相比，α-生育酚组和ALA组的尿8OHdG浓度均显著降低（P<0.001），但尿8OHdG浓度的最终平均水平仅在α-生育酚组和安慰剂组之间存在显著差异（P<0.01）。
在DS儿童饮食中补充α-生育酚可能会减轻DNA水平的氧化应激。
