Li Wen, Estrada-de los Santos Paulina, Matthijs Sandra, Xie Guan-Lin, Busson Roger, Cornelis Pierre, Rozenski Jef, De Mot René
Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Heverlee-Leuven 3001, Belgium.
Chem Biol. 2011 Oct 28;18(10):1320-30. doi: 10.1016/j.chembiol.2011.08.006.
Under control of the Gac regulatory system, Pseudomonas putida RW10S1 produces promysalin to promote its own swarming and biofilm formation, and to selectively inhibit many other pseudomonads, including the opportunistic pathogen Pseudomonas aeruginosa. This amphipathic antibiotic is composed of salicylic acid and 2,8-dihydroxymyristamide bridged by a unique 2-pyrroline-5-carboxyl moiety. In addition to enzymes for salicylic acid synthesis and activation, the biosynthetic gene cluster encodes divergent type II fatty acid biosynthesis components, unusual fatty acid-tailoring enzymes (two Rieske-type oxygenases and an amidotransferase), an enzyme resembling a proline-loading module of nonribosomal peptide synthetases, and the first prokaryotic member of the BAHD family of plant acyltransferases. Identification of biosynthetic intermediates enabled to propose a pathway for synthesis of this bacterial colonization factor.
在Gac调控系统的控制下，恶臭假单胞菌RW10S1产生前莫萨林以促进自身群体运动和生物膜形成，并选择性抑制许多其他假单胞菌，包括机会致病菌铜绿假单胞菌。这种两亲性抗生素由水杨酸和2,8-二羟基肉豆蔻酰胺通过独特的2-吡咯啉-5-羧基部分连接而成。除了水杨酸合成和激活所需的酶外，生物合成基因簇还编码不同类型的II型脂肪酸生物合成成分、不寻常的脂肪酸修饰酶（两种 Rieske 型加氧酶和一种酰胺转移酶）、一种类似于非核糖体肽合成酶脯氨酸装载模块的酶，以及植物酰基转移酶BAHD家族的首个原核成员。生物合成中间体的鉴定使得能够提出这种细菌定殖因子的合成途径。
