Ajinomoto Co., Inc., 15-1 Kyobashi 1-Chome, Chuo-ku, Tokyo 104-8315, Japan.
Food Chem Toxicol. 2011 Nov;49 Suppl 1:S49-59. doi: 10.1016/j.fct.2011.06.044.
Advantame (N-[N-[3-(3-hydroxy-4-methoxyphenyl) propyl]-α-aspartyl]-L-phenylalanine 1-methyl ester, monohydrate), an N-substituted analog of aspartame, has been developed as a high-intensity sweetener. Groups of 4 dogs of each sex were treated at 0, 2000, 10,000, or 50,000 ppm of advantame in the diet for 52 weeks. Additional groups of 2 dogs/sex at the control, and mid- and high-dose groups were treated for 52 weeks followed by a 6-week recovery period. There was no effect of treatment on mortality, body weight, organ weights, food consumption, or the results of ophthalmological, electrocardiographic, haematological, clinical chemistry or urinalysis examinations. No histopathological changes were associated with advantame treatment. The NOAEL was considered to be 50,000 ppm, the highest concentration tested, which was equivalent to 2057 and 2139 mg/kg body weight/day in males, and females, respectively. The results of the study support the safety of advantame for use as a high-intensity sweetener.
Advantame（N-[N-[3-（3-羟基-4-甲氧基苯基）丙基]-α-天冬氨酰]-L-苯丙氨酸 1-甲酯，一水合物），一种阿斯巴甜的 N-取代类似物，已被开发为高强度甜味剂。每组 4 只雌雄各半的狗分别以 0、2000、10000 或 50000ppm 的 advantame 剂量喂食饮食 52 周。对照组和中、高剂量组的每组 2 只狗/性别连续治疗 52 周，然后进行 6 周恢复期。处理对死亡率、体重、器官重量、食物消耗或眼科、心电图、血液学、临床化学或尿液分析检查结果均无影响。与 advantame 处理无关的组织病理学变化。NOAEL 被认为是 50000ppm，即测试的最高浓度，相当于雄性和雌性分别为 2057 和 2139mg/kg 体重/天。该研究结果支持 advantame 用作高强度甜味剂的安全性。
