Roche Molecular Systems, 4300 Hacienda Drive, Pleasanton, CA, USA.
J Clin Virol. 2011 Dec;52 Suppl 1:S77-82. doi: 10.1016/j.jcv.2011.09.015. Epub 2011 Oct 28.
Significant advancements in molecular diagnostics have been made since the inception and application of PCR-based technologies in clinical diagnostic laboratories and the management of HIV-1 infected patients. More recently, real-time PCR has improved the overall performance of assays used for detecting and quantifying HIV-1 RNA viral load in patients undergoing antiretroviral treatment. The effects of these changes and the interpretations of the HIV-1 viral load results are discussed in this review in the context of the different assays used, the viral dynamics of the HIV-1 virus, and the recent changes to HIV-1 treatment guidelines.
自 PCR 技术在临床诊断实验室中的应用和 HIV-1 感染患者的管理以来，分子诊断技术取得了重大进展。最近，实时 PCR 提高了用于检测和定量接受抗逆转录病毒治疗的患者的 HIV-1 RNA 病毒载量的检测的整体性能。在本文中，我们将讨论这些变化的影响以及 HIV-1 病毒载量结果的解释，同时还将讨论不同检测方法的应用、HIV-1 病毒的病毒动力学以及 HIV-1 治疗指南的最新变化。
