Department of Animal Science and Technology, School of Bioresource and Bioscience, Chung-Ang University, Ansung, Gyeonggi-Do, Republic of Korea.
Reprod Toxicol. 2011 Dec;32(4):418-24. doi: 10.1016/j.reprotox.2011.09.011. Epub 2011 Oct 20.
Xenoestrogenic compounds (XCs) can disrupt endogenous hormone function and affect sperm function by binding to receptors on sperm membrane. Albeit spermatozoa are potentially a useful model for screening estrogenic activities of endocrine disruptors, high-quality in vitro test system that examination of the XCs effects on sperm function is required. The objective of this study was to compare the effects of XCs (genistein and 4-tert-octylphenol) to those of steroids (estrogen and progesterone) and heparin on in vitro capacitation and acrosome reaction (AR) in mouse spermatozoa. Mouse spermatozoa were incubated with various concentrations (0.001-100 μM) of each chemical for 15 or 30 min, and then capacitation and AR were assessed using chlortetracycline. All chemicals studied effectively alter capacitation and/or AR in mouse spermatozoa with different manner. Therefore, we believed that our system will provide a good in vitro model system to characterize the physiological effect of XCs especially when compared with steroids.
类雌激素化合物 (XCs) 可以通过与精子膜上的受体结合来破坏内源性激素功能并影响精子功能。尽管精子是筛选内分泌干扰物雌激素活性的潜在有用模型，但需要一种高质量的体外测试系统来检查 XCs 对精子功能的影响。本研究的目的是比较 XCs（染料木黄酮和 4-叔辛基苯酚）与甾体（雌激素和孕酮）和肝素对体外获能和顶体反应 (AR) 的影响老鼠精子。将精子与各种浓度（0.001-100 μM）的每种化学物质孵育 15 或 30 分钟，然后使用金霉素评估获能和 AR。所有研究的化学物质都能有效地改变老鼠精子的获能和/或 AR，方式不同。因此，我们相信我们的系统将提供一个很好的体外模型系统来描述 XCs 的生理效应，特别是与甾体相比时。
