Cancer Research UK London Research Institute, London, UK.
FEBS Lett. 2011 Dec 15;585(24):3850-5. doi: 10.1016/j.febslet.2011.10.033. Epub 2011 Oct 25.
The MCM (mini-chromosome maintenance) complex is the core of the eukaryotic replicative helicase and comprises six proteins, Mcm2-Mcm7. In humans, a variant form of the complex has Mcm2 replaced by the MCM-BP protein. Recent results suggest that a similar complex exists in fission yeast with an essential role in DNA replication and cell cycle progression. Here, we describe the purification and subunit composition of the fission yeast MCM(Mcb1) complex. Using newly generated temperature-sensitive alleles, we show that loss of MCM(Mcb1) function leads to accumulation of DNA damage, checkpoint activation and cell cycle arrest, and provide evidence for a role for MCM(Mcb1) in meiosis.
MCM（微染色体维持）复合物是真核复制解旋酶的核心，由六个蛋白组成，分别是 Mcm2-Mcm7。在人类中，复合物的一种变体形式将 Mcm2 替换为 MCM-BP 蛋白。最近的结果表明，裂殖酵母中存在一种类似的复合物，在 DNA 复制和细胞周期进程中具有重要作用。在这里，我们描述了裂殖酵母 MCM（Mcb1）复合物的纯化和亚基组成。使用新生成的温度敏感等位基因，我们表明 Mcm（Mcb1）功能的丧失导致 DNA 损伤的积累、检查点的激活和细胞周期的停滞，并提供了 Mcm（Mcb1）在减数分裂中作用的证据。
