Department of Obstetrics and Gynecology, Kyung Hee University Hospital, Kyung Hee University, School of Medicine, Seoul, Republic of Korea.
Biochem Biophys Res Commun. 2011 Nov 18;415(2):401-4. doi: 10.1016/j.bbrc.2011.10.081. Epub 2011 Oct 20.
In most animals, somatic cell centrosomes are inherited from the centriole of the fertilizing spermatozoa. The oocyte centriole degenerates during oogenesis, and completely disappears in metaphase II. Therefore, the embryos generated by in vitro parthenogenesis are supposed to develop without any centrioles. Exceptional acentriolar and/or acentrosomal developments are possible in mice and in some experimental cells; however, in most animals, the full developmental potential of parthenogenetic cells in vitro and the fate of their centrioles/centrosomes are not clearly understood. To predict the future of in vitro human parthenogenesis, we explored the centrioles/centrosomes in ovarian mature cystic teratoma cells by immunofluorescent staining and transmission electron microscopy. We confirmed the presence of centrioles and centrosomes in these well-known parthenogenetic ovarian tumor cells. Our findings clearly demonstrate that, even without a sperm centriole, parthenotes that develop from activated oocytes can produce their own centrioles/centrosomes, and can even develop into the well-differentiated mature tissue.
在大多数动物中，体细胞中心体是从受精精子的中心粒中遗传而来的。卵母细胞中心粒在卵母细胞发生过程中退化，并在中期 II 完全消失。因此，体外孤雌生殖产生的胚胎应该在没有任何中心粒的情况下发育。在老鼠和一些实验细胞中，可能会出现例外的无中心粒和/或无中心体发育；然而，在大多数动物中，体外孤雌生殖细胞的完全发育潜能及其中心粒/中心体的命运尚不清楚。为了预测体外人类孤雌生殖的未来，我们通过免疫荧光染色和透射电子显微镜探索了卵巢成熟囊性畸胎瘤细胞中的中心粒/中心体。我们证实了这些众所周知的孤雌生殖卵巢肿瘤细胞中存在中心粒和中心体。我们的发现清楚地表明，即使没有精子中心粒，从激活的卵母细胞发育而来的孤雌胚胎也可以产生自己的中心粒/中心体，甚至可以发育成分化良好的成熟组织。
