酵母单杂交检测用于基因中心的人类基因调控网络作图。
Yeast one-hybrid assays for gene-centered human gene regulatory network mapping.
机构信息
Program in Systems Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
出版信息
Nat Methods. 2011 Oct 30;8(12):1050-2. doi: 10.1038/nmeth.1764.
Abstract
Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.
摘要
适用于 Gateway 载体的酵母单杂交（Y1H）检测方法为鉴定能够与目的 DNA 序列结合的转录因子提供了一种便利的基因中心（从 DNA 到蛋白质）方法。我们提供了 Y1H 资源，包括 1434 个预测的人类转录因子中的 988 个（69%）克隆，可用于检测人类 DNA 区域和转录因子之间的已知和新的相互作用。
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