Olchowik Grażyna, Chadaj-Polberg Emilia, Tomaszewski Marek, Polberg Mateusz, Tomaszewska Monika
Department of Biophysics, Medical University of Lublin, Poland.
Folia Histochem Cytobiol. 2011;49(3):504-11. doi: 10.5603/fhc.2011.0071.
The influence of pregnancy on bone tissue metabolism is not completely understood. Caffeine also has a potentially negative influence on bones. The aim of this study was the evaluation of changes in the bones of pregnant rats under the influence of caffeine. The experiment was carried out on Wistar rats. The evaluation of rats' bone tissue quality was performed based on bone density measurements and resistance examinations. It analyzed the impact of caffeine on the degree of bone tissue mineralization and the composition of the bones. The mean value of pelvises 'wet' and 'dry' densities in a group of pregnant rats with caffeine intake was lower compared to the control group. The deformation in maximal load point of the femur shaft in the experimental group was significantly higher than in the control group. In the experimental group, the percentage of water in the bones was significantly higher, while the content of inorganic phase was significantly lower compared to the control group. The changes of biomechanical parameters in the group of pregnant rats with caffeine intake indicate its negative influence on the bone. Our results show higher plasticization of the bone shafts of the animals under the influence of caffeine. Higher deformation of bone shafts may have an effect on the statics of the skeleton. The administration of caffeine significantly affected the quantitative composition of the bone.
妊娠对骨组织代谢的影响尚未完全明确。咖啡因对骨骼也可能有负面影响。本研究旨在评估咖啡因影响下妊娠大鼠骨骼的变化。实验选用Wistar大鼠进行。基于骨密度测量和抗性检查对大鼠骨组织质量进行评估。分析了咖啡因对骨组织矿化程度和骨骼成分的影响。与对照组相比，摄入咖啡因的妊娠大鼠组骨盆“湿”密度和“干”密度的平均值较低。实验组股骨干最大负荷点处的变形明显高于对照组。实验组骨骼中的水分百分比明显更高，而与对照组相比，无机相含量明显更低。摄入咖啡因的妊娠大鼠组生物力学参数的变化表明其对骨骼有负面影响。我们的结果显示，在咖啡因影响下动物的骨干有更高的可塑性。骨干更高的变形可能会影响骨骼的静力学。咖啡因的给药显著影响了骨骼的定量组成。
