星形胶质细胞衍生的微血管内皮细胞中的 stathmin 表达：一个新的治疗靶点。
Stathmin expression in glioma-derived microvascular endothelial cells: a novel therapeutic target.
机构信息
Department of Neurosurgery, the Fourth Affiliated Hospital of Harbin Medical University, and Department of Pathophysiology, Harbin Medical University, Harbin 150001, PR China.
出版信息
Oncol Rep. 2012 Mar;27(3):714-8. doi: 10.3892/or.2011.1525. Epub 2011 Oct 31.
The purpose of this study was to investigate stathmin expression and its mechanisms of action in GDMEC. Microvascular endothelial cells were isolated from human gliomas (n=68) and normal brain specimans (n=20), and purified by magnetic beads coated with anti-CD105 antibody. The expression of stathmin mRNA and protein were detected by RT-PCR and western blotting, respectively. Stathmin expression was silenced by application of specific siRNA in high grade GDMEC. The proliferation, apoptosis and invasion behavior of GDMEC were investigated. The stathmin positive rate of endothelial cells in normal brain, grade I-II glioma and grade III-IV glioma was 20, 66 and 95.5%, respectively (P<0.05). When cells were treated with siRNA to silence stathmin, cell viability was reduced, the apoptosis rate increased and the migration of vascular endothelial cells was suppressed significantly (P<0.05). Down-regulation of stathmin suppressed neoangiogenesis of glioma and provides a potential target for glioma treatment.
本研究旨在探讨 stathmin 在 GDMEC 中的表达及其作用机制。从人胶质瘤（n=68）和正常脑组织标本（n=20）中分离微血管内皮细胞，并通过涂有抗 CD105 抗体的磁珠进行纯化。通过 RT-PCR 和 Western blot 分别检测 stathmin mRNA 和蛋白的表达。应用特异性 siRNA 沉默高等级 GDMEC 中的 stathmin 表达。研究 GDMEC 的增殖、凋亡和侵袭行为。正常脑组织、I- II 级胶质瘤和 III-IV 级胶质瘤中内皮细胞的 stathmin 阳性率分别为 20%、66%和 95.5%（P<0.05）。当用 siRNA 沉默 stathmin 时，细胞活力降低，凋亡率增加，血管内皮细胞的迁移明显受到抑制（P<0.05）。下调 stathmin 抑制了神经胶质瘤的新生血管形成，为神经胶质瘤的治疗提供了一个潜在的靶点。
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