Jang Kuem Hee, Hwang Ui Wook
School of Life Science, Graduate School, Kyungpook National University, Daegu 702-701, Korea.
Mitochondrial DNA. 2011 Aug;22(4):77-9. doi: 10.3109/19401736.2011.624601.
The black-headed snake Sibynophis collaris (Reptilia, Squamata, Colubridae) is a least concern species in the world. Two universal and two specific polymerase chain reaction (PCR) primers were used for long PCRs to amplify the whole mitochondrial genome of S. collaris. The products were subjected to do sequencing reactions. The complete genome is 17,163 bp in size, containing 37 genes coding for 13 proteins, 2 rRNAs, 22 tRNAs, and 2 control regions (CRI and CRII). The results could play an important role in the preservation of genetic resources for helping conservation of the endangered species.
黑头剑蛇（爬行纲，有鳞目，游蛇科）在世界上属于无危物种。使用两对通用引物和两对特异性聚合酶链反应（PCR）引物进行长片段PCR，以扩增黑头剑蛇的整个线粒体基因组。对产物进行测序反应。完整基因组大小为17,163 bp，包含37个基因，编码13种蛋白质、2个核糖体RNA、22个转运RNA以及2个控制区（CRI和CRII）。这些结果对于遗传资源保护，进而帮助濒危物种的保护可能具有重要作用。
