Clinical Embryology, Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, India.
Andrologia. 2012 May;44 Suppl 1:642-9. doi: 10.1111/j.1439-0272.2011.01243.x. Epub 2011 Nov 1.
With increasing evidence for faulty paternal contribution to reproduction, there has been a steady increase in studies highlighting an association between sperm DNA damage, failed/delayed fertilisation and aberrant embryo development. Owing to prevailing ambiguity, the aims of the study were to analyse the genetic integrity of the male gamete and then to understand its association with age, standard semen parameters, lifestyle and occupational factors. The study included 504 subjects, attending university infertility clinic for fertility evaluation and treatment. Semen characteristics were analysed by standard criteria; terminal deoxynucelotidyl transferase-mediated nick end-labelling assay was employed for DNA damage assessment. The average incidence of sperm DNA damage in patients with normozoospermic semen parameters was <10%. Patients with oligozoospermia, severe oligozoospermia, oligoasthenoteratospermia, asthenoteratozoospermia and necrozoospermia had significantly higher level of sperm DNA damage (P < 0.001). Patients above 40 years of age had significantly high levels of DNA damage (P < 0.001) compared with their counterparts. Patients with varicocele and a history of alcohol consumption had higher incidence of spermatozoa with DNA damage (P < 0.01). Poor sperm characteristics in the ejaculate are associated with increased sperm DNA damage. Age-related increase in sperm DNA damage and association of the same with varicocele and alcohol consumption are also demonstrated.
随着越来越多的证据表明父系在生殖方面存在缺陷，越来越多的研究强调了精子 DNA 损伤、受精失败/延迟以及胚胎发育异常之间的关联。由于普遍存在的模糊性，本研究的目的是分析男性配子的遗传完整性，然后了解其与年龄、标准精液参数、生活方式和职业因素的关系。该研究纳入了 504 名在大学不孕不育诊所接受生育评估和治疗的患者。精液特征采用标准标准进行分析；末端脱氧核苷酸转移酶介导的 nick 末端标记法用于评估 DNA 损伤。在具有正常精子参数的患者中，精子 DNA 损伤的平均发生率<10%。少精子症、严重少精子症、少弱精子症、弱畸形精子症和死精子症患者的精子 DNA 损伤水平显著升高（P<0.001）。与对照组相比，年龄>40 岁的患者 DNA 损伤水平显著升高（P<0.001）。精索静脉曲张和饮酒史患者的精子 DNA 损伤发生率更高（P<0.01）。精液中精子特征较差与精子 DNA 损伤增加有关。还证明了与年龄相关的精子 DNA 损伤增加以及与精索静脉曲张和饮酒的关联。
