Department of Microbiology, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Arch Oral Biol. 2012 May;57(5):491-4. doi: 10.1016/j.archoralbio.2011.10.009. Epub 2011 Oct 29.
Interferon-gamma (IFN-γ) is highly expressed in oral lichen planus (OLP). The IFN-γ (+874 in intron 1, rs2430561) TT genotype, which has been reported to be associated with high IFN-γ production, was hypothesized to be associated with susceptibility to OLP in the Thai population.
Genomic DNA samples from 74 OLP and 268 healthy controls were evaluated for IFN-γ polymorphisms by polymerase chain reaction with sequence-specific primers (PCR-SSP) and direct sequencing method.
The T allele was significantly associated with an increased risk of OLP development as compared to the A allele (OR=1.76, P=0.004; P(c)=0.02). The effect of the T allele was similar to an autosomal recessive disorder; the presence of TT genotype (when compared to AA and AT) conferred an OR of 2.61, P=0.008; P(c)=0.04.
We found an association between IFN-γ +874A/T polymorphism and susceptibility to OLP. However, an association study utilising a larger sample size and patients from other races apart from the Asian population should be performed to further verify these findings.
干扰素-γ（IFN-γ）在口腔扁平苔藓（OLP）中高度表达。据报道，IFN-γ（+874 在内含子 1，rs2430561）TT 基因型与高 IFN-γ 产生有关，假设与泰国人群的 OLP 易感性有关。
通过聚合酶链反应与序列特异性引物（PCR-SSP）和直接测序方法评估来自 74 例 OLP 和 268 例健康对照的基因组 DNA 样本中的 IFN-γ 多态性。
与 A 等位基因相比，T 等位基因与 OLP 发病风险增加显著相关（OR=1.76，P=0.004；P(c)=0.02）。T 等位基因的作用类似于常染色体隐性疾病；与 AA 和 AT 相比，TT 基因型的存在（OR=2.61，P=0.008；P(c)=0.04）赋予了 2.61 的 OR。
我们发现 IFN-γ+874A/T 多态性与 OLP 易感性之间存在关联。然而，应该进行更大样本量的关联研究，并对除亚洲人群以外的其他种族的患者进行研究，以进一步验证这些发现。
