Petersen-Bjørn S, Harrington T R, Friesen J D
Hospital for Sick Children, Toronto, Ontario, Canada.
Yeast. 1990 Jul-Aug;6(4):345-52. doi: 10.1002/yea.320060407.
ORF2 is an essential gene immediately upstream of PRP4 (formerly RNA4), a gene involved in nuclear mRNA processing in Saccharomyces cerevisiae. The two genes are arranged head-to-head. An 8 base-pair conserved sequence element is found upstream of both genes, as well as upstream of certain other genes that are known to be involved in pre-mRNA processing. Through deletion analysis we have found that both of the conserved sequence elements are important for transcription of both genes. We have cloned ORF2 and have isolated temperature-sensitive orf2 mutants. The phenotype of these mutants does not suggest a role for ORF2 in mRNA processing. The deduced amino acid sequence of ORF2 indicates significant similarity to DPR1, a gene encoding a protein that is involved in the carboxy-terminal processing of G-protein.
ORF2是PRP4（原RNA4）上游紧邻的一个必需基因，PRP4是酿酒酵母中参与核mRNA加工的基因。这两个基因呈头对头排列。在这两个基因的上游以及某些已知参与前体mRNA加工的其他基因的上游，发现了一个8个碱基对的保守序列元件。通过缺失分析，我们发现这两个保守序列元件对两个基因的转录都很重要。我们克隆了ORF2，并分离出了温度敏感型orf2突变体。这些突变体的表型并未表明ORF2在mRNA加工中起作用。ORF2推导的氨基酸序列与DPR1有显著相似性，DPR1是一个编码参与G蛋白羧基末端加工的蛋白质的基因。
