Department of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00161, Rome, Italy.
Langmuir. 2011 Dec 20;27(24):15048-53. doi: 10.1021/la202912f. Epub 2011 Nov 16.
The concept that the effective unit of interest in the cell-nanomaterial interaction is the particle and its corona of associated proteins is emerging. Here we investigate the compositional evolution of the protein corona of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) cationic liposomes (CLs) and DOTAP/DNA lipoplexes over a wide range of plasma concentrations (2.5-80%). The composition of the hard corona of lipoplexes is quite stable, but that of CLs does evolve considerably. We show that the protein corona of CLs is made of both low-affinity and competitive-binding proteins whose relative abundance changes with the plasma concentration. This result may have deep biological implications for the application of lipid-based gene vectors both in vitro and in vivo.
细胞-纳米材料相互作用中感兴趣的有效单元是颗粒及其相关蛋白质的冠层，这一概念正在出现。在这里，我们研究了在广泛的血浆浓度（2.5-80%）范围内，1,2-二油酰基-3-三甲铵丙烷（DOTAP）阳离子脂质体（CL）和 DOTAP/DNA 脂质体的蛋白质冠层的组成演变。脂质体的硬冠层组成相当稳定，但 CL 的组成变化很大。我们表明，CL 的蛋白质冠层由低亲和力和竞争结合蛋白组成，其相对丰度随血浆浓度而变化。这一结果可能对脂类基因载体在体外和体内的应用具有深远的生物学意义。
