Trinh Duong N, Radlinskaite Meda, Cheeseman Jack, Kuhnle Gunter, Osborn Helen M I, Meleady Paula, Spencer Daniel I R, Monopoli Marco P
Department of Chemistry, Royal College of Surgeons in Ireland, RCSI University of Medicine and Health Sciences, D02 YN77 Dublin, Ireland.
School of Physics & Clinical & Optometric Sciences, Technological University Dublin, D07 EWV4 Dublin, Ireland.
Nanomaterials (Basel). 2022 Aug 3;12(15):2661. doi: 10.3390/nano12152661.
Biomolecular corona is spontaneously formed on the surface of nanoparticles (NPs) when they are in contact with biological fluids. It plays an important role in the colloidal stability of NPs, which is of importance for most of their medical applications and toxicity assessment. While typical studies use either blood plasma or serum from a pooled biobank, it is unclear whether differences in the media, such as cholesterol level or protein concentration, might affect the NP colloidal stability and corona composition. In this study, the silica corona was prepared at particularly low plasma concentrations (3%, /-1.98 mg/mL) to identify the critical roles of the protein mass/NP surface ratio and the level of plasma cholesterol on the corona protein pattern and particle stability. While depending on the plasma dilution factor, the corona protein composition could be controlled by keeping the protein/NP constant. The NP colloidal stability was found to strongly correlate with the level of cholesterol in human plasma, particularly due to the high enrichment of high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in the corona. A cohort study on plasma samples from individuals with known cholesterol levels was performed to highlight that association, which could be relevant for all corona systems enriched with the LDL.
当纳米颗粒（NPs）与生物流体接触时，生物分子冠会自发形成于其表面。它在NPs的胶体稳定性中起着重要作用，这对其大多数医学应用和毒性评估都很重要。虽然典型研究使用来自集中生物样本库的血浆或血清，但尚不清楚诸如胆固醇水平或蛋白质浓度等介质差异是否会影响NP胶体稳定性和冠层组成。在本研究中，以特别低的血浆浓度（3%，±1.98 mg/mL）制备二氧化硅冠层，以确定蛋白质质量/NP表面比和血浆胆固醇水平对冠层蛋白质模式和颗粒稳定性的关键作用。虽然取决于血浆稀释因子，但通过保持蛋白质/NP恒定可以控制冠层蛋白质组成。发现NP胶体稳定性与人体血浆中的胆固醇水平密切相关，特别是由于冠层中高密度脂蛋白（HDL）和低密度脂蛋白（LDL）的高度富集。对来自已知胆固醇水平个体的血浆样本进行了队列研究，以突出这种关联，这可能与所有富含LDL的冠层系统相关。
