Katsu T, Kuroko M, Morikawa T, Sanchika K, Yamanaka H, Shinoda S, Fujita Y
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Biochim Biophys Acta. 1990 Aug 24;1027(2):185-90. doi: 10.1016/0005-2736(90)90083-z.
Mastoparan-induced changes in the K+ permeability of rat peritoneal mast cells, human erythrocytes, Staphylococcus aureus and Escherichia coli were examined. Mastoparan did not efficiently increase the K+ permeability of cells except for S. aureus. The release of membrane phospholipids was also observed from S. aureus cells in the concentration range of the permeability enhancement. Mastoparan stimulated histamine release from mast cells, independently of a small efflux of K+. Mastoparan became markedly effective to E. coli cells whose outer membrane structure was chemically disrupted beforehand, showing that the peptide can enhance the permeability of the cytoplasmic membranes of both Gram-positive and -negative bacteria. In experiments using liposomes, mastoparan increased the permeability of the liposomes composed of egg phosphatidylethanolamine and egg phosphatidylglycerol, which are the lipid constituents of the cytoplasmic membrane of E. coli cells, while it showed a weak activity to the liposomes composed of egg phosphatidylcholine and cholesterol. The latter result related closely to the fact that this peptide acted weakly on erythrocytes and mast cells in which acidic lipids constitute a minor portion. Mastoparan decreased the phase transition temperature of dipalmitoylphosphatidylglycerol liposomes, but it did not affect that of dipalmitoylphosphatidylcholine liposomes. These results indicate that mastoparan penetrated into membranes mainly containing acidic phospholipids and disrupted the membrane structure to increase the permeability. The action of the wasp venom mastoparan was compared with that of a bee venom melittin.
研究了马斯托帕兰对大鼠腹膜肥大细胞、人红细胞、金黄色葡萄球菌和大肠杆菌钾离子通透性的影响。除金黄色葡萄球菌外，马斯托帕兰不能有效增加其他细胞的钾离子通透性。在通透性增强的浓度范围内，也观察到金黄色葡萄球菌细胞膜磷脂的释放。马斯托帕兰刺激肥大细胞释放组胺，与少量钾离子外流无关。马斯托帕兰对预先经化学方法破坏其外膜结构的大肠杆菌细胞有显著作用，表明该肽可增强革兰氏阳性菌和革兰氏阴性菌细胞质膜的通透性。在使用脂质体的实验中，马斯托帕兰增加了由大肠杆菌细胞质膜脂质成分卵磷脂乙醇胺和卵磷脂甘油组成的脂质体的通透性，而对由卵磷脂胆碱和胆固醇组成的脂质体活性较弱。后一结果与该肽对酸性脂质占比小的红细胞和肥大细胞作用较弱这一事实密切相关。马斯托帕兰降低了二棕榈酰磷脂酰甘油脂质体的相变温度，但不影响二棕榈酰磷脂酰胆碱脂质体的相变温度。这些结果表明，马斯托帕兰主要穿透到含有酸性磷脂的膜中，破坏膜结构以增加通透性。比较了黄蜂毒液马斯托帕兰和蜜蜂毒液蜂毒肽的作用。
