Ariga T, Kusunoki S, Asano K, Oshima M, Asano M, Mannen T, Yu R K
Department of Membrane Biochemistry, Tokyo Metropolitan Institute of Medical Science, Japan.
Brain Res. 1990 Jun 11;519(1-2):57-64. doi: 10.1016/0006-8993(90)90060-o.
Sulfated glucuronyl glycolipids (SGGLs) in human dorsal root ganglion (DRG) and sympathetic ganglion (SG) were analyzed biochemically and immunohistochemically. SGGLs were enriched in human DRG (1.02 +/- 0.23 micrograms/mg protein), whereas much lower concentrations of these glycolipids (0.043 +/- 0.23 micrograms/mg protein) were detected in SG. Myelin within DRG and SG was immunostained by anti-SGGL antiserum, although only a few myelinated fibers were seen in SG. Nerve cell bodies or unmyelinated fibers were not immunostained. Subcellular fractionation study of human DRG demonstrated that these glycolipids were not only enriched in myelin but also in the axolemma-enriched fraction. These data are consistent with the view that SGGLs may be expressed on myelinated fibers in myelin and axolemma, suggesting that these compounds may play an important role in regulating myelinogenesis.
对人背根神经节（DRG）和交感神经节（SG）中的硫酸化葡萄糖醛酸糖脂（SGGLs）进行了生化和免疫组织化学分析。SGGLs在人DRG中含量丰富（1.02±0.23微克/毫克蛋白质），而在SG中检测到的这些糖脂浓度要低得多（0.043±0.23微克/毫克蛋白质）。抗SGGL抗血清对DRG和SG中的髓鞘进行了免疫染色，尽管在SG中仅可见少数有髓纤维。神经细胞体或无髓纤维未被免疫染色。对人DRG的亚细胞分级分离研究表明，这些糖脂不仅在髓鞘中富集，而且在富含轴膜的组分中也有富集。这些数据与SGGLs可能在髓鞘和轴膜的有髓纤维上表达的观点一致，表明这些化合物可能在调节髓鞘形成中起重要作用。
