Kohriyama T, Kusunoki S, Ariga T, Yoshino J E, DeVries G H, Latov N, Yu R K
J Neurochem. 1987 May;48(5):1516-22. doi: 10.1111/j.1471-4159.1987.tb05694.x.
Peripheral nerve glycolipids, with which anti-myelin-associated glycoprotein (MAG) antibodies from patients with demyelinating neuropathy and plasma cell dyscrasia cross-react, proved to be novel glycosphingolipids containing a sulfated glucuronyl residue. Consequently, there has been much interest in the immunological role that these sulfated glucuronyl-glycosphingolipids (SGGLs) may play in the pathogenesis of this disorder. For the determination of the distribution of these glycolipids in various nervous tissues and, thereby, the elucidation of their pathogenicity, a quantitative immunostaining-TLC method for their detection has been devised. Using this method, we demonstrated that these glycolipids were distributed in greatly different amounts in the peripheral nerves from human, bovine, chicken, rat, and rabbit. Subcellular localization studies of bovine peripheral nerve also demonstrated that they were enriched in the axolemma-enriched fraction and present in glial-related membranes in lower concentrations. In addition, these glycolipids were present in bovine dura mater and transformed rat Schwann cells. These biochemical results suggest that not only myelin but also axons could be involved as targets of the anti-MAG antibody in macroglobulinemia neuropathy, and it may also be necessary to examine anti-SGGL activity in patients with axonal neuropathy associated with plasma cell dyscrasia.
周围神经糖脂可与脱髓鞘性神经病和浆细胞发育异常患者的抗髓鞘相关糖蛋白（MAG）抗体发生交叉反应，结果证明它是一种含有硫酸化葡糖醛酸残基的新型糖鞘脂。因此，人们对这些硫酸化葡糖醛酸糖鞘脂（SGGLs）在该疾病发病机制中可能发挥的免疫作用非常感兴趣。为了确定这些糖脂在各种神经组织中的分布，从而阐明它们的致病性，已经设计了一种用于检测它们的定量免疫染色-TLC方法。使用这种方法，我们证明这些糖脂在人、牛、鸡、大鼠和兔的周围神经中的分布量差异很大。对牛周围神经的亚细胞定位研究还表明，它们在富含轴膜的部分中富集，而在神经胶质相关膜中的浓度较低。此外，这些糖脂存在于牛硬脑膜和转化的大鼠雪旺细胞中。这些生化结果表明，在巨球蛋白血症性神经病中，不仅髓鞘而且轴突都可能作为抗MAG抗体的靶标，并且可能还需要检查与浆细胞发育异常相关的轴索性神经病患者的抗SGGL活性。
