Brouwer J, Smekens F
Diagnostic Centre SSDZ, Delft, The Netherlands.
Clin Chim Acta. 1990 Aug 15;189(2):173-80. doi: 10.1016/0009-8981(90)90089-b.
An enzyme immunoassay (EIA) has been developed for the determination of alpha 1-antitrypsin (AT). The assay can measure AT in fecal extracts (0.2 g wet feces/ml) from all healthy individuals. Purified human AT was coupled to peroxidase by means of a heterobifunctional reagent and the conjugate was used as a labeled antigen in competitive immunoassays. Concentrations of AT in feces from healthy individuals were less than 0.55 mg/g wet weight or less than 2.2 mg/g dry weight. CV for the physiological day-to-day variance varied between 9 and 140%. After incubation for 48 h at 37 degrees C, the average recovery rates for AT were 78% in ileostomy fluids, 88% in fecal extracts and 92% in feces. Results obtained with EIA correlated well with those obtained with a commercial radial immunodiffusion assay (r = 0.95).
已开发出一种酶免疫测定法（EIA）用于测定α1-抗胰蛋白酶（AT）。该测定法可测量所有健康个体粪便提取物（0.2克湿粪便/毫升）中的AT。通过异双功能试剂将纯化的人AT与过氧化物酶偶联，该偶联物用作竞争性免疫测定中的标记抗原。健康个体粪便中AT的浓度低于0.55毫克/克湿重或低于2.2毫克/克干重。生理每日变化的CV在9%至140%之间。在37℃孵育48小时后，回肠造口液中AT的平均回收率为78%，粪便提取物中为88%，粪便中为92%。EIA获得的结果与商业放射免疫扩散测定法获得的结果相关性良好（r = 0.95）。
