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地衣芽孢杆菌BLAR传感转导蛋白羧基末端结构域在大肠杆菌中表达为一种水溶性的分子量26,000的青霉素结合蛋白。

Expression in Escherichia coli of the carboxy terminal domain of the BLAR sensory-transducer protein of Bacillus licheniformis as a water-soluble Mr 26,000 penicillin-binding protein.

作者信息

Joris B, Ledent P, Kobayashi T, Lampen J O, Ghuysen J M

机构信息

Université de Liège, Institut de Chimie, Belgium.

出版信息

FEMS Microbiol Lett. 1990 Jun 15;58(1):107-13. doi: 10.1016/0378-1097(90)90111-3.

DOI:10.1016/0378-1097(90)90111-3
PMID:2204571
Abstract

A cloning vector has been constructed which allows production and export by Escherichia coli of the Met346-Arg601 carboxy terminal domain of the 601 amino acid BLAR sensory-transducer involved in beta-lactamase inducibility in Bacillus licheniformis. The polypeptide, referred to as BLAR-CTD, accumulates in the periplasm of E. coli in the form of a water-soluble, Mr 26,000 penicillin-binding protein. These data and homology searches suggest that BLAR has a membrane topology similar to that of other sensory-transducers involved in chemotaxis.

摘要

已构建了一种克隆载体，它能使大肠杆菌产生并输出地衣芽孢杆菌中参与β-内酰胺酶诱导性的601个氨基酸的BLAR传感转导蛋白的Met346-Arg601羧基末端结构域。该多肽称为BLAR-CTD，以一种水溶性的、分子量为26,000的青霉素结合蛋白的形式积累在大肠杆菌的周质中。这些数据和同源性搜索表明，BLAR的膜拓扑结构与其他参与趋化性的传感转导蛋白相似。

相似文献

1
Expression in Escherichia coli of the carboxy terminal domain of the BLAR sensory-transducer protein of Bacillus licheniformis as a water-soluble Mr 26,000 penicillin-binding protein.地衣芽孢杆菌BLAR传感转导蛋白羧基末端结构域在大肠杆菌中表达为一种水溶性的分子量26,000的青霉素结合蛋白。
FEMS Microbiol Lett. 1990 Jun 15;58(1):107-13. doi: 10.1016/0378-1097(90)90111-3.
2
Identification of BlaR, the signal transducer for beta-lactamase production in Bacillus licheniformis, as a penicillin-binding protein with strong homology to the OXA-2 beta-lactamase (class D) of Salmonella typhimurium.地衣芽孢杆菌中β-内酰胺酶产生的信号转导蛋白BlaR被鉴定为一种青霉素结合蛋白，与鼠伤寒沙门氏菌的OXA-2β-内酰胺酶（D类）具有高度同源性。
J Bacteriol. 1990 Feb;172(2):1137-41. doi: 10.1128/jb.172.2.1137-1141.1990.
3
A second regulatory gene, blaR1, encoding a potential penicillin-binding protein required for induction of beta-lactamase in Bacillus licheniformis.第二个调控基因blaR1，编码地衣芽孢杆菌中诱导β-内酰胺酶所需的一种潜在青霉素结合蛋白。
J Bacteriol. 1987 Sep;169(9):3873-8. doi: 10.1128/jb.169.9.3873-3878.1987.
4
The penicillin sensory transducer, BlaR, involved in the inducibility of beta-lactamase synthesis in Bacillus licheniformis is embedded in the plasma membrane via a four-alpha-helix bundle.参与地衣芽孢杆菌中β-内酰胺酶合成诱导性的青霉素传感转导器BlaR通过一个四α-螺旋束嵌入质膜中。
Mol Microbiol. 1997 Mar;23(5):935-44. doi: 10.1046/j.1365-2958.1997.2761642.x.
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Domain organization of penicillin-binding protein 5 from Escherichia coli analysed by C-terminal truncation.通过C末端截短分析大肠杆菌青霉素结合蛋白5的结构域组织。
Biochem J. 1993 Jan 15;289 ( Pt 2)(Pt 2):593-8.
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Nucleotide sequences of the penicillin-binding protein 5 and 6 genes of Escherichia coli.大肠杆菌青霉素结合蛋白5和6基因的核苷酸序列。
Nucleic Acids Res. 1988 Feb 25;16(4):1617. doi: 10.1093/nar/16.4.1617.
7
AmpC and AmpH, proteins related to the class C beta-lactamases, bind penicillin and contribute to the normal morphology of Escherichia coli.AmpC和AmpH是与C类β-内酰胺酶相关的蛋白质，它们结合青霉素并有助于大肠杆菌的正常形态。
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Conversion of the alpha component of penicillin-binding protein 1b to the beta component in Escherichia coli.大肠杆菌中青霉素结合蛋白1b的α组分向β组分的转化。
J Bacteriol. 1987 Feb;169(2):891-3. doi: 10.1128/jb.169.2.891-893.1987.
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A gene fusion that localises the penicillin-binding domain of penicillin-binding protein 3 of Escherichia coli.一种使大肠杆菌青霉素结合蛋白3的青霉素结合结构域定位的基因融合。
FEBS Lett. 1984 Oct 15;176(1):179-84. doi: 10.1016/0014-5793(84)80936-9.
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A water-soluble form of penicillin-binding protein 2 of Escherichia coli constructed by site-directed mutagenesis.通过定点诱变构建的大肠杆菌青霉素结合蛋白2的水溶性形式。
FEBS Lett. 1987 Dec 21;226(1):150-4. doi: 10.1016/0014-5793(87)80569-0.

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Analysis of the stability and affinity of BlaR-CTD protein to β-lactam antibiotics based on docking and mutagenesis studies.基于对接和诱变研究分析BlaR-CTD蛋白对β-内酰胺抗生素的稳定性和亲和力。
J Biol Eng. 2019 Mar 29;13:27. doi: 10.1186/s13036-019-0157-4. eCollection 2019.
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Bacillus licheniformis BlaR1 L3 loop is a zinc metalloprotease activated by self-proteolysis.地衣芽孢杆菌 BlaR1 L3 环是一种锌金属蛋白酶，通过自我水解激活。
PLoS One. 2012;7(5):e36400. doi: 10.1371/journal.pone.0036400. Epub 2012 May 18.
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Transcription of the gene mediating methicillin resistance in Staphylococcus aureus (mecA) is corepressed but not coinduced by cognate mecA and beta-lactamase regulators.介导金黄色葡萄球菌对甲氧西林耐药性的基因（mecA）的转录受到同源mecA和β-内酰胺酶调节因子的共抑制，但未被共诱导。
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blaI and blaR1 regulate beta-lactamase and PBP 2a production in methicillin-resistant Staphylococcus aureus.blaI和blaR1调控耐甲氧西林金黄色葡萄球菌中β-内酰胺酶和PBP 2a的产生。
Antimicrob Agents Chemother. 1993 May;37(5):1144-9. doi: 10.1128/AAC.37.5.1144.
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Comparison of the sequences of class A beta-lactamases and of the secondary structure elements of penicillin-recognizing proteins.A类β-内酰胺酶序列与青霉素识别蛋白二级结构元件的比较。
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