Ono B, Ishino-Arao Y, Takasugi K, Taniguchi M, Fukuda M, Fukui M, Miyakawa I, Sando N
Laboratory of Environmental Hygiene Chemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Genetics. 1990 Aug;125(4):729-38. doi: 10.1093/genetics/125.4.729.
A mutant of Saccharomyces cerevisiae representing a novel life cycle, named "alternative self-diploidization" or "ASD" homothallism, was obtained fortuitously. In this life cycle, MAT alpha (or MATa) haplophase and MAT alpha/MAT alpha (or MATa/MATa) diplophase alternate. Germinated cells are haploid and mating. They soon become nonmating and sporogenous as they vegetatively grow. They sooner or later diploidize presumably via endomitosis. The diploid cells haploidize via normal meiosis. A single recessive nuclear mutation, named asd 1-1, is responsible for "ASD" homothallism. In the rho 0 cytoplasm, asd 1-1 cells mate even if at a low efficiency and fail to diploidize. Since pet mutations do not have such effects, we conclude that a certain mitochondrial function other than respiration is required for manifestation of "ASD" homothallism. That is, "ASD" homothallism is the result of some sort of nuclear-cytoplasmic interaction.
偶然获得了一种酿酒酵母突变体，它代表一种新的生命周期，称为“交替自体二倍体化”或“ASD”同宗配合。在这个生命周期中，MATα（或MATa）单倍体阶段和MATα/MATα（或MATa/MATa）二倍体阶段交替出现。萌发的细胞是单倍体且可交配。随着它们营养生长，很快变得不可交配并产生孢子。它们迟早可能通过核内有丝分裂二倍体化。二倍体细胞通过正常减数分裂单倍体化。一个单一的隐性核突变，命名为asd 1-1，导致“ASD”同宗配合。在ρ0细胞质中，asd 1-1细胞即使交配效率低也能交配，但不能二倍体化。由于pet突变没有这种效应，我们得出结论，“ASD”同宗配合的表现需要呼吸作用以外的某种线粒体功能。也就是说，“ASD”同宗配合是某种核质相互作用的结果。
