Department of Gastroenterology, Beijing Army General Hospital, Beijing 100700, China.
World J Gastroenterol. 2011 Sep 21;17(35):3986-93. doi: 10.3748/wjg.v17.i35.3986.
To explore the dynamic changes of prion protein (PrPc) in the process of gastric cancer drug resistance and the role of PrPc expression in the prognosis of gastric cancer patients receiving chemotherapy.
A series of gastric cancer cell lines resistant to different concentrations of adriamycin was established, and the expression of PrPc, Bcl-2 and Bax was detected in these cells. Apoptosis was determined using Annexin V staining. Western blotting and immunohistochemistry were performed to detect the expression of PrPc in patients receiving chemotherapy and to explore the role of PrPc expression in predicting the chemosensitivity and the outcome of gastric cancer patients receiving chemotherapy. Follow-up was performed for 2 years.
PrPc expression was increased with the increase in drug resistance. Bcl-2, together with PrPc, increased the level of anti-apoptosis of cancer cells. Increased PrPc expression predicted the enhanced level of anti-apoptosis and resistance to anticancer drugs. PrPc expression could be used as a marker for predicting the efficacy of chemotherapy and the prognosis of gastric cancer. Increased PrPc expression predicted both poor chemosensitivity and a low 2-year survival rate. Contrarily, low PrPc expression predicted favorable chemosensitivity and a relatively high 2-year survival rate.
PrPc expression is associated with histological types and differentiation of gastric cancer cells; The PrPc expression level might be a valuable marker in predicting the efficacy of chemotherapy and the prognosis of gastric cancer patients receiving chemotherapy.
探讨朊蛋白（PrPc）在胃癌耐药过程中的动态变化及 PrPc 表达在接受化疗的胃癌患者预后中的作用。
建立一系列对不同浓度阿霉素耐药的胃癌细胞系，检测这些细胞中 PrPc、Bcl-2 和 Bax 的表达。用 Annexin V 染色法检测细胞凋亡。采用 Western blot 和免疫组织化学法检测接受化疗的患者中 PrPc 的表达，探讨 PrPc 表达在预测胃癌患者化疗敏感性和预后中的作用。随访 2 年。
PrPc 表达随耐药程度的增加而增加。Bcl-2 与 PrPc 一起增加了癌细胞的抗凋亡水平。增加的 PrPc 表达预测了增强的抗凋亡水平和对抗癌药物的耐药性。PrPc 表达可作为预测化疗疗效和胃癌患者预后的标志物。增加的 PrPc 表达预测化疗敏感性差和 2 年生存率低。相反，低 PrPc 表达预测化疗敏感性好，2 年生存率相对较高。
PrPc 表达与胃癌细胞的组织学类型和分化有关；PrPc 表达水平可能是预测接受化疗的胃癌患者化疗疗效和预后的有价值标志物。
