Wong D H, Ignatius M J, Parosky G, Parham P, Trojanowski J Q, Brodsky F M
Department of Pharmacy, School of Pharmacy, University of California, San Francisco.
J Neurosci. 1990 Sep;10(9):3025-31. doi: 10.1523/JNEUROSCI.10-09-03025.1990.
High-molecular-weight forms of clathrin light chains LCa and LCb contain inserted sequences and are expressed in brain tissue but have not been observed in peripheral tissues. Monoclonal antibodies specific for the high-molecular-weight form of LCb and all forms of LCa were used to analyze their expression in different species and different neuronal cell types. High-molecular-weight light chains were found in bovine, rat, mouse, chicken, and human brain, indicating a conserved pattern of expression. Neuron-specific expression of the high-molecular-weight light chains was suggested by analysis of human brain gray matter and white matter. The former contained a higher proportion of light chains with insertion sequences. Immunohistochemical analysis localized the high-molecular-weight form of LCb to synapses and neuronal perikarya, but not to glial cells. Immunofluorescent labeling of cultured chicken dorsal root ganglia confirmed expression in neurons but not Schwann cells. These results indicate that the high-molecular-weight forms of clathrin light chains are restricted in expression and found in neuronal cells.
网格蛋白轻链LCa和LCb的高分子量形式含有插入序列，在脑组织中表达，但在外周组织中未观察到。针对LCb的高分子量形式和所有形式的LCa的单克隆抗体被用于分析它们在不同物种和不同神经元细胞类型中的表达。在牛、大鼠、小鼠、鸡和人类大脑中发现了高分子量轻链，表明存在保守的表达模式。通过对人类脑灰质和白质的分析，提示了高分子量轻链的神经元特异性表达。前者含有较高比例的带有插入序列的轻链。免疫组织化学分析将LCb的高分子量形式定位到突触和神经元胞体，但未定位到神经胶质细胞。对培养的鸡背根神经节的免疫荧光标记证实了在神经元而非施万细胞中的表达。这些结果表明，网格蛋白轻链的高分子量形式在表达上受到限制，并存在于神经元细胞中。
