Brodsky F M, Parham P
J Mol Biol. 1983 Jun 15;167(1):197-204. doi: 10.1016/s0022-2836(83)80041-2.
Two types of light chains were observed in clathrins from different bovine tissues. Clathrin was purified from seven different bovine tissues using immunoaffinity columns prepared from a monoclonal antibody specific for the heavier light chain (LCa) of bovine brain clathrin. The LCa equivalent in clathrin from non-brain tissue was identified by monoclonal antibody binding to electrophoretic blots of these clathrins. The LCa and LCb light chains of bovine brain clathrin are, respectively, 4 X 10(3) daltons and 3 X 10(3) daltons heavier than their corresponding light chains in other tissues. The light chains from tissues other than brain are similar in molecular weight.
在来自不同牛组织的网格蛋白中观察到两种类型的轻链。使用由针对牛脑网格蛋白重轻链（LCa）的单克隆抗体制备的免疫亲和柱，从七种不同的牛组织中纯化网格蛋白。通过单克隆抗体与这些网格蛋白的电泳印迹结合，鉴定非脑组织中网格蛋白中的等效LCa。牛脑网格蛋白的LCa和LCb轻链分别比其他组织中相应的轻链重4×10³道尔顿和3×10³道尔顿。来自脑以外组织的轻链分子量相似。
