Zeng LiHua, Ji XiTuan, Zhang YanJun, Miao Xia, Zou ChangXu, Lang HaiYang, Zhang Jie, Li YuRong, Wang XiaoWu, Qi HongXing, Ren DongQin, Guo GuoZhen
Department of Radiation Medicine, The Fourth Military Medical University, Xi'an, China.
Electromagn Biol Med. 2011 Dec;30(4):205-18. doi: 10.3109/15368378.2011.587929.
Male Sprague Dawley rats were exposed to EMP irradiation of 100 kV/m peak-to-peak e-field intensity and different numbers of pulses. Rat sperm samples were prepared for analysis of sperm qualities; Testes were assessed by transmission electron microscopy and serum hormone concentrations were examined by radioimmunoassay; Enzymatic activities of Total-superoxide dismutase(T-SOD) and manganese-superoxide dismutase (MnSOD), the mRNA levels of MnSOD and cuprozinc-superoxide dismutase (CuZnSOD), and the density of malondialdehyde (MDA) were also determined. EMP irradiation did not affect spermatozoon morphology, micronucleus formation rate, sperm number or viability, but the acrosin reaction rate decreased at 24 h and 48 h and recovered by 72 h after irradiation as compared to the controls. The ultrastructure of rat testis displayed more serious damage at 24 h than at other time points (6 h, 12 h, 48 h). Serum levels of luteotrophic hormone (LH) and testosterone (T) were elevated in irradiated rats as compared to controls. After irradiation, enzymatic activities of T-SOD and MnSOD were reduced by 24 h, consistent with the changes observed in MnSOD mRNA expression; MDA content increased at 6 h in turn. These studies have quantified the morphological damage and dysfunction in the rat reproductive system induced by EMP. The mechanism of EMP induced damage may be associated with the inhibition of MnSOD expression.
将雄性Sprague Dawley大鼠暴露于峰峰值电场强度为100 kV/m的电磁脉冲（EMP）辐射下，并给予不同的脉冲数。制备大鼠精子样本以分析精子质量；通过透射电子显微镜评估睾丸，并通过放射免疫测定法检测血清激素浓度；还测定了总超氧化物歧化酶（T-SOD）和锰超氧化物歧化酶（MnSOD）的酶活性、MnSOD和铜锌超氧化物歧化酶（CuZnSOD）的mRNA水平以及丙二醛（MDA）的含量。EMP辐射不影响精子形态、微核形成率、精子数量或活力，但与对照组相比，辐射后24小时和48小时顶体酶反应率降低，72小时后恢复。大鼠睾丸的超微结构在24小时时比其他时间点（6小时、12小时、48小时）显示出更严重的损伤。与对照组相比，受辐射大鼠血清中的促黄体生成素（LH）和睾酮（T）水平升高。辐射后，T-SOD和MnSOD的酶活性在24小时时降低，这与MnSOD mRNA表达的变化一致；MDA含量在6小时时依次增加。这些研究已经量化了EMP对大鼠生殖系统造成的形态损伤和功能障碍。EMP诱导损伤的机制可能与MnSOD表达受抑制有关。
