Laboratório de Toxicologia Ambiental, LABTOX, Depto. de Engenharia Sanitária e Ambiental, Universidade Federal de Santa Catarina, Campus Universitário, CEP: 88040-970, Caixa Postal 476, Florianópolis, SC, Brasil.
Ecotoxicol Environ Saf. 2012 Feb;76(2):63-70. doi: 10.1016/j.ecoenv.2011.10.016. Epub 2011 Nov 1.
In this study, lipid peroxidation and DNA methylation were observed in erythrocytes of Oreochromis niloticus exposed to soluble fractions of textile, metal-mechanic and pulp and paper industrial waste, after a period of 48 h. Lipid peroxidation was quantified by the rate of malondialdehyde (MDA) and DNA methylation was quantified by the rate of 5-methyldeoxycytosine (m⁵dC). Soluble fractions of textile industrial waste caused metabolic changes for all studied samples. In organisms exposed to samples TX1 and TX2 (textile waste) MDA rates were 132.36 and 140.28 nM MDA/mg protein, respectively, while in control organism the MDA rates were 27.5 nM MDA/mg protein. All samples from soluble fractions of textile industrial waste induced increases in m⁵dC rates, increases varied between 300 percent and 700 percent when compared to the control organism. All the organisms exposed to soluble fractions of metal-mechanic industrial waste presented increases between 360 percent and 600 percent in the rates of MDA, and one of them (sample MM3) induced an increase of 180 percent in the rate of m⁵dC, when compared to control. Although a significant increase was not observed in the MDA rate of fish exposed to the soluble fractions of pulp and paper industrial waste, there was an increase of 460 percent in the rate of m⁵dC in one of the samples (sample PP2), when compared to control. The results showed that the soluble fractions of these industrial wastes are capable of inducing oxidative damage and altering the DNA methylation of O. niloticus. Thus, the MDA and m⁵dC rates demonstrated to be effective biomarkers of exposure, which could be used to evaluate the toxicity of soluble fractions of industrial solid waste.
在这项研究中，观察到暴露于纺织、金属机械和纸浆造纸工业废物的可溶部分 48 小时后，奥利亚罗非鱼的红细胞中发生脂质过氧化和 DNA 甲基化。通过丙二醛（MDA）的速率来量化脂质过氧化，通过 5-甲基脱氧胞嘧啶（m⁵dC）的速率来量化 DNA 甲基化。纺织工业废物的可溶部分导致所有研究样本发生代谢变化。在暴露于 TX1 和 TX2（纺织废物）样品的生物体中，MDA 速率分别为 132.36 和 140.28 nM MDA/mg 蛋白，而在对照生物中，MDA 速率为 27.5 nM MDA/mg 蛋白。纺织工业废物的可溶部分的所有样品均诱导 m⁵dC 速率增加，与对照生物相比，增加幅度在 300%至 700%之间。暴露于金属机械工业废物的可溶部分的所有生物体的 MDA 速率均增加了 360%至 600%，其中一个（样品 MM3）的 m⁵dC 速率增加了 180%，与对照相比。尽管暴露于纸浆造纸工业废物的可溶部分的鱼的 MDA 速率没有显著增加，但在一个样品（样品 PP2）中，m⁵dC 速率增加了 460%，与对照相比。结果表明，这些工业废物的可溶部分能够诱导氧化损伤并改变奥利亚罗非鱼的 DNA 甲基化。因此，MDA 和 m⁵dC 速率被证明是暴露的有效生物标志物，可用于评估工业固体废物可溶部分的毒性。
