Pierre S, Tarnowska K, Hachfi L, Coupé S, Simide R, Couvray S, Garnier C, Grimaldi M, Richard S, Gaillard S, Prévot-D'Alvise N, Grillasca J P
Équipe de Biologie Moléculaire Marine-PROTEE, Université du Sud Toulon-Var, BP 20132, Avenue de l'Université, 83957 La Garde Cedex, France.
Cell Mol Biol (Noisy-le-grand). 2011 Oct 28;57 Suppl:OL1614-22.
It has been previously demonstrated that "Warm temperature Acclimation-related 65 kD Protein" (WAP65) is involved in temperature acclimation, response to intoxication and infection, as well as in development. The expression of wap65-1 was investigated in the liver of European sea bass (Dicentrarchus labrax) during exposure to the increased temperature (from 12 deg C to 30 deg C) and during intoxication with four heavy metals: lead, cadmium, copper and zinc. Post temperature increase wap65 expression was highest after one hour at 30 deg C. After 1 to 4 weeks at 30 deg C wap65 transcript levels did not differ from the 12 deg C control group, similar to observations regarding the heat shock protein, hsp70. Upregulation of wap65 was detected after treatment (intoxication) with cadmium (0.5 μg/l). In contrast, a slight, but significant down regulation of wap65 was seen after copper (5 μg/l) intoxication. These data indicate that functional analyses of WAP65 are needed to understand the differential regulation of this gene by metals. The role of WAP65 may be similar to that of HSP70, which has generalized functions in responding to certain stressors and maintaining normal cell physiology.
先前已经证明，“与温度适应相关的65kD蛋白”（WAP65）参与温度适应、对中毒和感染的反应以及发育过程。研究了欧洲海鲈（Dicentrarchus labrax）肝脏中wap65-1在温度升高（从12℃升至30℃）期间以及在接触四种重金属（铅、镉、铜和锌）中毒期间的表达情况。温度升高后，wap65在30℃下1小时后的表达最高。在30℃下1至4周后，wap65转录水平与12℃对照组无差异，这与热休克蛋白hsp70的观察结果相似。在用镉（0.5μg/l）处理（中毒）后检测到wap65上调。相反，在用铜（5μg/l）中毒后，wap65出现轻微但显著的下调。这些数据表明，需要对WAP65进行功能分析，以了解该基因受金属的差异调控。WAP65的作用可能与HSP70相似，HSP70在应对某些应激源和维持正常细胞生理方面具有广泛的功能。
