Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research, P.O. Box 2214, 710 03 Iraklio, Crete, Greece.
Mol Phylogenet Evol. 2010 May;55(2):640-9. doi: 10.1016/j.ympev.2009.10.001. Epub 2009 Oct 21.
The warm temperature acclimation protein Wap65 has been shown to be involved in temperature acclimation, in immune response as well as in development. In teleosts, two types of Wap65 proteins, Wap65-1 and Wap65-2 are found, both acting as a multifunctional agent in several biological processes. In the present study we identified both transcripts Wap65-1 and Wap65-2 for the European seabass (Dicentrarchus labrax), examined their evolutionary rate and performed selection tests. The two paralogues were shown to be under moderate positive selection indicating their evolutionary adaptation. This functional diversification was further explored through expression studies. Both transcripts were differentially expressed during development as well as in various tissues and pathogen challenges, showing that Wap65-1 and Wap65-2 have evolved diverse functions. These results direct to the hypothesis that Wap65 proteins may, similarly to heat-shock proteins, have a general role in cell physiology.
暖温驯化蛋白 Wap65 已被证明参与温度驯化、免疫反应以及发育过程。在硬骨鱼类中，发现了两种类型的 Wap65 蛋白，Wap65-1 和 Wap65-2，它们在多种生物学过程中都作为多功能因子发挥作用。在本研究中，我们鉴定了欧洲鲈鱼（Dicentrarchus labrax）的 Wap65-1 和 Wap65-2 两种转录本，研究了它们的进化率并进行了选择测试。这两个基因的直系同源物受到适度的正选择，表明它们在进化上适应了环境。通过表达研究进一步探索了这种功能多样化。这两种转录本在发育过程以及在各种组织和病原体挑战中都有差异表达，表明 Wap65-1 和 Wap65-2 已经进化出不同的功能。这些结果使我们假设 Wap65 蛋白可能与热休克蛋白类似，在细胞生理学中具有普遍作用。
