Lasko D D, Tomkinson A E, Lindahl T
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts., Great Britain.
Mutat Res. 1990 Sep-Nov;236(2-3):277-87. doi: 10.1016/0921-8777(90)90011-s.
Recent studies on eukaryotic DNA ligases are briefly reviewed. The two distinguishable enzymes from mammalian cells, DNA ligase I and DNA ligase II, have been purified to homogeneity and characterized biochemically. Two distinct DNA ligases have also been identified in Drosophila melanogaster embryos. The genes encoding DNA ligases from Schizosaccharomyces pombe, Saccharomyces cerevisiae and vaccinia virus have been cloned and sequenced. These 3 proteins exhibit about 30% amino acid sequence identity; the 2 yeast enzymes share 53% amino acid sequence identity or conserved changes. Altered DNA ligase I activity has been found in cell lines from patients with Bloom's syndrome, although a causal link between the enzyme deficiency and the disease has not yet been proven.
本文简要综述了近期关于真核生物DNA连接酶的研究。从哺乳动物细胞中分离出两种不同的酶，即DNA连接酶I和DNA连接酶II，并对其进行了纯化和生化特性鉴定。在果蝇胚胎中也鉴定出两种不同的DNA连接酶。粟酒裂殖酵母、酿酒酵母和痘苗病毒中编码DNA连接酶的基因已被克隆和测序。这三种蛋白质的氨基酸序列一致性约为30%；两种酵母酶的氨基酸序列一致性或保守性变化为53%。在布卢姆综合征患者的细胞系中发现了DNA连接酶I活性的改变，尽管尚未证实酶缺陷与该疾病之间存在因果关系。
