Barsov Eugene V
AIDS and Cancer Virus Program, SAIC-Frederick, Inc., Frederick, Maryland, USA.
Curr Protoc Immunol. 2011 Nov;Chapter 7:Unit 7.21B. doi: 10.1002/0471142735.im0721bs95.
Human and rhesus macaque primary antigen-specific T cells derived from infected or immunized individuals or animals are a valuable material with which to study cellular immune responses against pathogens and tumors. Antigen-specific T cells can be expanded in vitro but have a finite proliferative life span. After a limited period in culture, primary T cells undergo replicative senescence and stop dividing. This restricts their applicability to short-term experiments and complicates their use in adoptive immunotherapy. The proliferative life span of primary human and rhesus macaque T cells can be considerably extended by ectopically expressed human telomerase reverse transcriptase (TERT). Antigen-specific T cells transduced with TERT-expressing retroviral vectors can proliferate and expand in culture for long periods of time while maintaining their primary T cell characteristics, including antigen-specific responses. Thus, TERT-immortalized T cells are an important and valuable resource for studying T cell-mediated immune responses and, potentially, for adoptive immunotherapy.
源自受感染或免疫的个体或动物的人源和恒河猴原代抗原特异性T细胞是研究针对病原体和肿瘤的细胞免疫反应的宝贵材料。抗原特异性T细胞可以在体外扩增，但具有有限的增殖寿命。在培养有限时间后，原代T细胞会经历复制性衰老并停止分裂。这限制了它们在短期实验中的适用性，并使其在过继免疫治疗中的应用变得复杂。通过异位表达人端粒酶逆转录酶（TERT），人源和恒河猴原代T细胞的增殖寿命可以显著延长。用表达TERT的逆转录病毒载体转导的抗原特异性T细胞可以在培养中长时间增殖和扩增，同时保持其原代T细胞特征，包括抗原特异性反应。因此，TERT永生化T细胞是研究T细胞介导的免疫反应以及潜在的过继免疫治疗的重要且有价值的资源。
