Yunnan NO.2 Provincial People's Hospital, Kunming, China.
Int J Exp Pathol. 2011 Dec;92(6):377-81. doi: 10.1111/j.1365-2613.2011.00786.x. Epub 2011 Nov 3.
This study investigated the reactive changes in Müller glial cells and astrocytes of the rat retinae, which had been subjected either to hypoxia or to hypoxia followed by hyperoxia treatments. Fifteen rats were used. Ten rats were exposed to 9% O(2) for 2 h. Of these, five rats were killed at 24 h later; the remaining five rats were immediately exposed to 80% O(2) for 2 h and then killed 24 h later. Double immunofluorescence was carried out between nestin and glutamine synthetase (GS) and between glial fibrilary acidic proteins (GFAP) and GS in normal and pathological retinae. Enhanced nestin expression was observed in reactive astrocytes following hypoxia treatment as revealed in whole mount sections. A novel finding was the induction of nestin expression in Müller glial cells. Remarkably, the nestin immunostaining was downregulated to levels comparable to those of the normal rats with immediate hyperoxia treatment. Induced nestin expression by hypoxia colabelled with GFAP in astrocytes, however, remained unaffected after hyperoxia treatment. The induced expression of nestin in Müller glial cells and astrocytes in hypoxia and differential downregulation after hyperoxia treatment suggest a structural plasticity of the cytoskeletal framework of these cells. The differential response after hyperoxia treatment may be related to the functional states of the cells.
本研究探讨了经历缺氧或缺氧后复氧处理的大鼠视网膜中 Müller 胶质细胞和星形胶质细胞的反应性变化。使用了 15 只大鼠。10 只大鼠暴露于 9%的 O(2)中 2 小时。其中，5 只大鼠在 24 小时后处死；其余 5 只大鼠立即暴露于 80%的 O(2)中 2 小时，然后在 24 小时后处死。在正常和病理视网膜中进行巢蛋白与谷氨酰胺合成酶 (GS) 之间以及神经胶质纤维酸性蛋白 (GFAP) 与 GS 之间的双重免疫荧光。在全铺片切片中观察到缺氧处理后反应性星形胶质细胞中增强的巢蛋白表达。一个新的发现是 Müller 胶质细胞中巢蛋白表达的诱导。值得注意的是，立即进行复氧处理可将巢蛋白免疫染色下调至与正常大鼠相当的水平。然而，缺氧诱导的巢蛋白表达与星形胶质细胞中的 GFAP 共标，在复氧处理后不受影响。缺氧诱导的 Müller 胶质细胞和星形胶质细胞中巢蛋白的表达以及复氧处理后的差异下调表明这些细胞细胞骨架框架的结构可塑性。复氧处理后的差异反应可能与细胞的功能状态有关。
