Trowald-Wigh G, Thorén-Tolling K
Department of Medicine and Surgery, Veterinary Faculty, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Acta Vet Scand. 1990;31(1):87-95. doi: 10.1186/BF03547581.
A phagocytic function assay of canine granulocytes was established. This method allows the proportion of active granulocytes to be estimated as well as the number of adhered and ingested yeast cells. The influence of different factors on phagocytosis was studied. Temperature variation within the interval 36-41 degrees C did not affect phagocytosis. The incubation time for optimal phagocytosis of yeast cells was 35 min. The opsonization procedure giving the optimal phagocytosis was purified IgG and serum together.
建立了犬粒细胞的吞噬功能测定方法。该方法能够估计活性粒细胞的比例以及黏附并吞噬的酵母细胞数量。研究了不同因素对吞噬作用的影响。在36 - 41摄氏度区间内的温度变化不影响吞噬作用。酵母细胞最佳吞噬作用的孵育时间为35分钟。产生最佳吞噬作用的调理过程是纯化的IgG和血清共同作用。